Cloning and Bioinformatics Analysis of GABA A Receptor γ2 Subunit Gene in Carassais auratus gibebiol

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.028

Abstract

Abstract: The application of the specificity of GABA receptor subunits in drug screening and drug development got widely attention recently, of which three function subunits α1, β2 and γ2 studied deeply. Furthermore, Carassais auratus gibebiol as its good growth and reproduction had been widely farmed in china. In the present study, we cloned and characterized GABA A receptor γ2 subunit full-length gene. The full-length Carassais auratus gibebiol GABA A receptor γ2 subunit cDNA was 2 763 bp in length, contained a 1437 bp open reading frame(ORF), and encoded 477 amino acids which constituting a 55.3 kD protein molecule with an isoelectric point of 9.13. The γ2 subunit protein was hydrophilic. The protein sequence had one signal peptide, consisting of 35 amino acid residue. The sequence of amino acids contained four transmembrane regions, which length of 23，20，23 and 23 aa, involving in electronic transfer catalysis between the internal and external membrane. We found three N-glycosylation sites, two O- glycosylation sites and an extracellular domain which had obvious ligand-gated ion channels’ characteristics. Sequence comparison revealed that the similarity of the γ2 subunit protein all above 89% with other aquatic animals showed that it belongs to GABA A receptor subunits’ family. We conducted a phylogenetic analysis using the neighbor joining(NJ)method. The evolutionary tree showed that the γ2 subunit protein was clustered with the zebra fish which indicated that they are the two most closely related species.

Key words: Carassais auratus gibebiol, GABA-A receptor γ2 subunit, cloning, bioinformatics analysis

Zhao Yini, Hu Kun, Sun Qi, Yang Xianle, Ruan Jiming, Zhou Ailing. Cloning and Bioinformatics Analysis of GABA A Receptor γ2 Subunit Gene in Carassais auratus gibebiol[J]. Biotechnology Bulletin, 2015, 31(3): 191-198.

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