Effects of PDSW Combined with Hyperthermia on COX-2 and Bcl-2 Expressions in Gastric Cancer Cells and Tissues

doi:10.13560/j.cnki.biotech.bull.1985.2016-0691

Abstract

Abstract: This work aims to explore the effect of PDSW combined with hyperthermia on COX-2 and Bcl-2 expressions in gastric cancer cells SGC-7901 and tissues，and to clarify its anti-cancer effects on gastric cancer cells SGC-7901 in vitro and in vivo. The SGC-7901 cells were cultured in vitro and then were randomly divided into experimental，control and blank groups；the experimental group was added with PDSW，control group was given with normal saline，blank group was in no other treatment，and each group were cultured under 37℃，40℃ and 43℃ respectively. Concurrently，the SGC-7901 cells were injected into the nude mice subcutaneous to establish the transplantation tumor model. The injected mice were randomly divided into 5 groups，and then given the treatment with H2O+RT，H2O +40℃，H2O +43℃，PDSW+40℃，and PDSW+43℃ respectively when the tumor cells grew to 1 cm diameter. qRT-PCR and Western blot were used to detect the mRNA of gene COX-2 and Bcl-2 and the protein expression of COX-2 and Bcl-2 in the cells and tissues. As results，in SGC-7901 cells and nude mice subcutaneous transplantation tumor tissues，the expressions of gene COX-2 and Bcl-2 mRNA and their protein expressions decreased under the treatment of hyperthermia. Compared with the treatment of normal saline group，the expressions of gene COX-2 and Bcl-2 and their protein expression decreased remarkably under the treatment of PDSW combined with hyperthermia. Conclusively，PDSW combined with hyperthermia may inhibit the expressions of gene COX-2 and Bcl-2 and their protein expressions in gastric cancer SGC-7901 cells and tissues，so as to effectively inhibit the proliferation of gastric cancer cells.

Key words: PDSW, hyperthermia treats, SGC-7901, COX-2, Bcl-2, qRT-PCR, Western blot

TANG Hui-rong, CUI Jin ,ZHANG Jia-hua, DAI You-guo ,LI Wei-ming ,LIAO Chen. Effects of PDSW Combined with Hyperthermia on COX-2 and Bcl-2 Expressions in Gastric Cancer Cells and Tissues[J]. Biotechnology Bulletin, 2017, 33(7): 224-230.

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