Cloning,Expression and Bioinformatics Analyses of an ADC1 Gene in Hevea brasiliensis

doi:10.13560/j.cnki.biotech.bull.1985.2018-0498

Abstract

Abstract: Arginine decarboxylase（ADC）is an important enzyme involved in polyamine biosynthesis in higher plants. In order to study the expression and bioinformatics of ADC gene from rubber tree,HbADC1 from Hevea brasiliensis was cloned with PCR and sequencing methods. The sequence length of HbADC1 was 2 594 bpwith a 2 175 bp open reading frame,encoding 724 amino acids. Bioinformatics results indicated that the deduced molecular weight and isoelectric point of HbADC1 were 77.7 kD and 5.14,respectively. Phylogenetic analyses showed that HbADC1 and ADCs from Manihot esculenta and Jatropha curcas was clustered into one. qRT-PCR analyses demonstrated that HbADC1 expressed without tissue specificity,with the highest in female flowers,followed by stem apexes,male flowers,leaves,barks,and latex. HbADC1 expression significantly varied in different developmental stages of rubber tree leaf,with the highest expression in light young stage and the lowest one in mature stage,suggesting that HbADC1 might be associated with leaf development. In addition,HbADC1 expression were regulated by wounding,low temperature,drought,high salt,hydrogen peroxide,and ethylene treatments,indicating that HbADC1 might be involved in stress and ethylene responses in rubber tree.

Key words: Hevea brasiliensis, arginine decarboxylase, gene cloning, bioinformatics analysis, qRT-PCR

Hou Lan-fei, Yang Hong, Deng Zhi, Dai Long-jun, Men Zhong-hua, Li De-jun. Cloning,Expression and Bioinformatics Analyses of an ADC1 Gene in Hevea brasiliensis[J]. Biotechnology Bulletin, 2018, 34(11): 111-119.

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