Cloning,Expression and Comparative Analysis of Two Progesterone Receptor Genes in Cyprinus carpio

doi:10.13560/j.cnki.biotech.bull.1985.2018-1089

Abstract

Abstract: Progesterone receptor（Pgr）is a receptor mediating the regulation of progesterone on fish gonad development and germ cell maturation. To study the expression pattern and differential expression tendency of two common carp（Cyprinus carpio）Pgr genes,Pgr1 and Pgr2 were cloned using rapid amplification of cDNA ends. The full-length cDNA sequence lengths of Pgr1 and Pgr2 were 2 713 bp and 2 730 bp,both of which encoded proteins of 628 amino acids. They had a nucleotide identity of 91% and a protein identity of 90%. They both contained two domains,zinc finger domain and nuclear hormone receptor domain homologous superfamily. In the phylogenetic analysis,common carp Pgr1 and goldfish Pgr1 were clustered together and common carp Pgr2 and goldfish Pgr2 were grouped together. These two branches were then clustered with zebrafish Pgr. The ratio of the number of non-synonymous substitutions per non-synonymous site to the number of synonymous substitutions per synonymous site between Pgr1 and Pgr2 was 0.360,suggesting that they were under negative selection. qRT-PCR analysis demonstrated that Pgr1 and Pgr2 had higher expression levels in the gonads than in the other tissues,revealing that both genes might be associated with the gonad development of common carp. They had higher expression levels in sperms and oocytes than in embryos after induced by spawning drugs,indicating that they might be involved in germ cell maturation. In the most examined cases,significantly higher Pgr1 expression level was revealed compared with Pgr2,suggesting that Pgr1 plays the dominate role in common carp gonad development and germ cell maturation.

Key words: progesterone receptor（Pgr）, common carp, qRT-PCR, gonad, germ cell

ZHU Rui, YE Yu-qing, WANG Ya-xin, YANG Chen-ru, WANG Hong-wei, SUN Xiao-qing, ZHANG Yan, LI Shang-qi, LI Jiong-tang. Cloning,Expression and Comparative Analysis of Two Progesterone Receptor Genes in Cyprinus carpio[J]. Biotechnology Bulletin, 2019, 35(7): 46-53.

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