Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (6): 45-53.doi: 10.13560/j.cnki.biotech.bull.1985.2016-1085

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Advances in Gene Editing via Clustered Regularly Interspaced Short Palindromic Repeats

HAO Meng-yuan1, QU Xiao-jun2, CUI Yan-hua1   

  1. 1. School of Chemistry and Chemical Engineering,Harbin Institute of Technology,Harbin 150090;
    2. Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010
  • Received:2016-11-28 Online:2017-06-26 Published:2017-06-19

Abstract: CRISPR is a piece of clustered short-interspaced palindromic non-coding sequences,in which the invasive foreign DNA can be memorized by integrating new spacer sequences. However,Cas protein is responsible for supporting CRISPR transcription products---crRNA(CRISPR RNA)to splice foreign DNA. CRISPR-Cas system,based on the cooperation of the two segments,plays a role in resisting exogenous DNA infection. So far,CRISPR-Cas system attracts the attention from whole scientific community rapidly relying on its unique advantages,such as high efficiency,convenience,low cost and high specificity. This review summarizes the composition,structure and type of CRISPR-Cas system as well as the mechanism and advantages of CRISPR technology. Moreover,taking Streptococcus thermophilus as an example,the base arrangement and advanced structure of the CRISPR gene are also presented in order to provide references for the scientific research in molecular biology and related fields.

Key words: CRISPR-Cas system, gene editing, Streptococcus thermophilus, gRNA-Cas9 complex