The Influence of Exogenous Nitric Oxide on Microtubulin of Brassica pekinensis Under Drought Stress

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.023

Abstract

Abstract: In order to study the effect of nitricoxide(NO)on microtubulin in drought-stressed Brassica pekinensis, using B.pekinensis cultivar Jinyu-11 as experimental material, and the drought situation was simulated by treating 7-day seedlings with 15, 18, 21, 24, 27 g/mL of PEG6000 concentrations.Ultraviolet spectrophotometry and SDS-PAGE were used to investigate the differences of microtubulin content in cabbage seedling leaves, and the optimal treatment concentration of PEG6000 was determined to be 27 g/mL.Then the pretreated seedlings were fumigated with various concentrations of NO：50, 100, and 150 μmol/L respectively, and the variations of microtubulin content in the treated cabbages were observed.Results showed that the contents of microtubulin in cabbage seedling leaves demonstrated the trend of increasing with PEG6000 pre-treatments compared to control, but decreased after NO treatment.Moreover, both the content of endogenous NO and the expression of NO-related genes increased significantly under drought stress.Therefore, physiological concentration of NO alleviated the disaggregation of microtubulin caused from drought stress at certain level, which eliminated the damages to plants from the stress.

Key words: Brassica pekinensis, nitric oxide, drought stress, microtubulin

MA Xiao-li, JI Rui-ping. The Influence of Exogenous Nitric Oxide on Microtubulin of Brassica pekinensis Under Drought Stress[J]. Biotechnology Bulletin, 2016, 32(2): 172-177.

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