Cloning and Stress Response Analysis of PwRbcS in Picea wilsonii

doi:10.13560/j.cnki.biotech.bull.1985.2016.10.019

Abstract

Abstract: RbcS gene encodes a subunit of nuclear plant photosynthesis 1,5 two ribulose bisphosphate carboxylase / oxygenase. In this study,PwRbcS cDNA was obtained using RACE -PCR method. Bioinformatics analysis and real-time PCR technique were used to determine the relative expression of PwRbcS. The results showed that the full-length of PwRbcS cDNA was 936 bp,including the ORF of 552 bp encoding 186 amino acids. The theoretical molecular weight of PwRbcS is 20.712 6 kD and the pI value was 9.07. RT-qPCR showed that PwRbcS is mainly expressed in the needles,especially in mature needles. The expression of PwRbcS changed obviously under the treatment of NaCl,ABA and low temperature,respectively. After NaCl treatment,the expression level of PwRbcS changed significantly,reaching to 14 times than the control at 6 h. The expression level of PwRbcS reached to 24 times than the control after 8 h-ABA-treatment. And under low temperature stress,the expression increased gradually to 12 times than the control and followed by a drop,while under high temperature and PEG stress expression level did not change significantly. Therefore,PwRbcS may play an important role during abiotic stresses in planta.

Key words: Picea wilsonii, RbcS, bioinformatics, tissue expression, stress response

JU Dan, HU An-ni, ZHANG Jie, ZHANG Ling-yun. Cloning and Stress Response Analysis of PwRbcS in Picea wilsonii[J]. Biotechnology Bulletin, 2016, 32(10): 154-162.

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