cDNA Cloning，Sequence Analysis and Tissue Expression of Apaf-1 and Apaf-2 Gene of Goats

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.017

Abstract

Abstract: We collected the pituitary, ovary and other tissue samples of 5 Tibetan goats and 5 Jintang black goats at estrus stage, cloned cDNA of Apaf-1 and Apaf-2, analyzed their sequences, and investigated their mRNA expression of tissues by qPCR. The results showed that：the coding region sequence(CDS)of goat Apaf-1 gene was 3 750 bp long encoding 1 249 amino acids, and the CDS of Apaf-2 gene was 318 bp encoding 105 amino acids. The two genes showed the same sequences in both of goats, no mutation and no differential expression in 5 tissues. Our data suggested that gene Apaf-1 and Apaf-2 in animal's evolution were conservative, and the correlation with the trait of goat's prolificacy needs further studies.

Key words: goat, Apaf-1 gene, Apaf-2 gene, qPCR

Luo Bin, Lu Jianyuan, Ma Li, Hu Liang, Liu Shuang, Yang Kewei, Zi Xiangdong. cDNA Cloning，Sequence Analysis and Tissue Expression of Apaf-1 and Apaf-2 Gene of Goats[J]. Biotechnology Bulletin, 2015, 31(9): 125-130.

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