Methods of Measuring Telomere Length and Its Application

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.012

Abstract

Abstract: Aging and tumor are the two main factors influencing human longevity. Many studies have proved that telomere play important roles in these progresses. Thus, it is critical to familiarize with assay methods of telomere length. The most commonly used methods include telomere restriction fragment（TRF）length analysis, quantitative PCR（qPCR）, fluorescent in situ hybridization（FISH）and single telomere length analysis（STELA）. However, each method has the scope of application, in the process of research and application, we have to understand the principle of each method, its advantages and disadvantages, then we may choose the appropriate detection method to achieve the aim of our study and solve specific issues. The commonly used detection methods of telomere length are summarized here.

Key words: telomere length, TRF, qPCR, FISH, STELA

ZHANG Bin, WANG Chang-li. Methods of Measuring Telomere Length and Its Application[J]. Biotechnology Bulletin, 2016, 32(11): 93-98.

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