Effects of Organic Matters on the Abundance of Microorganisms in the Urban Wetland

doi:10.13560/j.cnki.biotech.bull.1985.2017-0528

Abstract

Abstract: Urban wetland suffers from not only the constant increase of organic matter load，but also the issue of organic matter in the topsoil is damaged in the process of urban development and construction. By adding the dissolved organic matter（DOM）solution of Cinnamomum camphora leaves，together with real-time fluorescence quantitative PCR，the effects of exogenous organic matter on the abundance of microorganisms in the topsoil（0-5 cm）was studied in simulative way. The results showed that changes in soil structure significantly affected the abundances of bacteria，archaea and functional genes associated with nitrogen cycles. The abundances of bacteria and functional genes associated with nitrogen cycles decreased significantly while soil organic matter content decreased（P <0.05）. The addition of nitrogen and DOM in the overlying water remarkably increased the abundances of microorganism and nitrogen functional genes in wetland. The exogenous DOM significantly affected the two genes in the transformation process of NO₂--N to N₂O and NO.

Key words: urban wetland, organic matter, qPCR, abundance of microorganism

XIE Jia, MA Xiao-hang, DAI Yan-ran, WU Juan, XIANG Dong-fang, CHENG Shui-ping. Effects of Organic Matters on the Abundance of Microorganisms in the Urban Wetland[J]. Biotechnology Bulletin, 2017, 33(10): 217-224.

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