Improvement and Optimization of Aptamer Selection

doi:10.13560/j.cnki.biotech.bull.1985.2017-0469

Abstract

Abstract: Aptamers have gained extensive attentions due to their similar function as antibody and many superior characteristics,how to use systematic evolution of ligands by exponential enrichment（SELEX）technology to efficiently screen aptamers is the key to its application. Combined with the research progress of aptamers in recent years,the article introduces optimization strategies for increasing the screening efficiency in three different stages of the screening process,including the design and modification of the initial random oligonucleotide library before SELEX,the selection method and generation of ssDNA library during SELEX,and the optimization of aptamer performance after SELEX（Post-SELEX）. Especially,the article compares many screening methods including capillary electrophoresis-SELEX,cell-SELEX,magnetic beads based-SELEX,tailored SELEX,microfluidic SELEX,microarray SELEX,and high-throughput SELE,as well as the methods for the generation of ssDNA such as asymmetric PCR,λ exonuclease digestion,magnetic beads-based separation,unequal length PCR,and some comprehensive methods. Also we explore the issues and prospects of the development of aptamers,providing references for related researchers.

Key words: aptamer, SELEX, screening efficiency, optimization strategy

LI Shi-yu, FU Qiang, YAN Ya-xian. Improvement and Optimization of Aptamer Selection[J]. Biotechnology Bulletin, 2017, 33(11): 67-75.

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