Abstract: Terpenoids are a large class of natural compounds which are synthesized from the precursor of 5-carbon isoprene unit,and they exist widely in plants,microorganisms and insects. Among them,monoterpene-like compounds are mainly used in production of flavors,cosmetics,food additives,pesticides,herbicides and advanced biofuels,i.e.,it presents wide application potential. In recent years,many terpenoid-producing recombinant Saccharomyces cerevisiae strains have been constructed,and the terpenoid yield is efficiently increased through approaches of metabolic engineering and synthetic biology. However,the synthesis of monoterpene by microorganism is relatively lagging behind because the lack of precursors,its toxicity to microorganisms,etc. limit the efficient synthesis of monoterpene. Here we discuss the recent advances of monoterpene synthesis in S. cerevisiae including the expression of monoterpene synthase,the enhancement of precursor geranyl pyrophosphate through dynamic regulation and the protein engineering,blocking the endogenous conversion of monoterpene,and improving the tolerance of the strains to monoterpene. There fore,we propose the feasible strategies toward resolving the bottleneck in microbial synthesis of monoterpene,aiming at providing potential references for optimizing the monoterpene-producing microbial cell factories.

Key words: monoterpene, Saccharomyces cerevisiae, geranyl pyrophosphate