Effects of Acid Mine Drainage on the Abundance of Functional Genes Involved in Nitrogen Cycle in Soil Profiles

doi:10.13560/j.cnki.biotech.bull.1985.2018-1094

Abstract

Abstract: Nitrogen（N）cycle in soil,which is mainly mediated by microorganisms,has always been being hotspots in environmental soil science and soil microbiology. Soil contamination significantly affects the course of N-cycle. The discharge of acid mining drainage（AMD）with low pH and containing high contents of salt and heavy metals generally leads to serious contamination of farmland soils. However,AMD’s influence on N-cycle in soil is rarely reported. A rice paddy contaminated by AMD in Tongling,Anhui province,was chosen as the research site. Based on the technique of molecular biology,the microbial biomass and the abundance of functional genes involved in N-cycle in soil profiles were detected,and main factors affecting the abundance of functional genes in soil profiles were analyzed. The results showed that the biomass of bacteria and archaea decreased with the increase of profile depth in the AMD-contaminated paddy soil. The abundance of functional genes involved in N-cycle was the highest in topsoil（0-10 cm）. In the studied soil profiles,the abundance of amoA-AOA gene was greater than that of amoA-AOB,and the abundance of nirK gene was greater than that of nirS gene,thus amoA-AOA and nirK were the dominated functional genes of ammonia oxidation and denitrification in the AMD-contaminated soil profile. Compared with uncontaminated soil,the abundance of nifH gene in the AMD-contaminated paddy soil profile significantly reduced,while the abundance of nirS gene significantly increased. pH was significantly and negatively correlated with the abundance of nirS,amoA-AOA and HZO genes. The iron content was extremely and negatively correlated with the biomass of bacteria and archaea and the abundance of nosZ,amoA-AOA,and HZO genes. In conclusion,there is a close relationship between the abundance of functional genes and both the profile depth and the physic-chemical characters of the rice paddy AMD-contaminated soil.

Key words: acid mine drainage, soil profile, nitrogen cycle, functional gene

LI Hui ZHA, Jian-jun, SUN Qing-ye. Effects of Acid Mine Drainage on the Abundance of Functional Genes Involved in Nitrogen Cycle in Soil Profiles[J]. Biotechnology Bulletin, 2019, 35(9): 249-256.

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