Screening and Identification of an Antagonistic Bacterium Against Rhizoctonia solani and Analysis of Biocontrol Factor

doi:10.13560/j.cnki.biotech.bull.1985.2019-0084

Abstract

Abstract: In order to screen the biocontrol bacteria against Rhizoctonia solani,plate confrontation method was used to screen 9 strains of bacteria with better bacteriostatic effect from potato rhizosphere soil. The strain Z17-2 with the best antibacterial activity was identified as Bacillus amyloliquefaciens based on morphological,physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA（GenBank accession number：MK271286）and gyrB（GenBank accession number：MK295695）sequences. Strain Z17-2 demonstrated antibacterial activity against Rhizoctonia solani,Alternaria solani,Fusarium oxysporum,Fusarium sambucinum,and Streptomyces scabie,while no inhibitory effect on Erwinia carotovora. By measuring the ability of producing lipopeptide,protease,cellulase,pectinase,amylase,iron carrier and phosphorus- and potassium-solubilizing,it was confirmed that strain Z17-2 contained lipopeptide-synthesized gene sfp,fenB,bacD and mycB,and produced protease,iron carrier,cellulase,while no pectinase,and presented no ability of dissolving phosphorus and potassium. The antibacterial ability of the lipopeptide extract in the toxic medium was determined to be 70.38%.

Key words: Rhizoctonia solani, Bacillus amyloliquefaciens, lipopeptide, biocontrol

SONG Ben-chao, ZHAO Dong-mei, YANG Zhi-hui, ZHANG Dai, ZHAO Zhi, ZHU Jie-hua. Screening and Identification of an Antagonistic Bacterium Against Rhizoctonia solani and Analysis of Biocontrol Factor[J]. Biotechnology Bulletin, 2019, 35(8): 9-16.

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