Correlation Between Genes Expression Levels of CaXMT1,CaDXMT1,CCS1 in Caffeine Biosynthesis Pathways and Caffeine Content in Coffee arabica

doi:10.13560/j.cnki.biotech.bull.1985.2019-0294

Abstract

Abstract: This work aims at exploring the correlation between expression levels of 3 key enzyme genes CaXMT1,CaDXMT1,CCS1 and caffeine synthesis in the biosynthetic pathways of caffeine in coffee（Coffee arabica）plants,as well as studying the synthetic mechanism of caffeine,thus providing theoretical evidences for the breeding of coffee trees. HPLC was used to measure the caffeine contents in the different tissues of coffee（C. arabica）plants at different developmental stages,as well as PCR（qRT-PCR）to measure the mRNA levels of CaXMT1,CaDXMT1 and CCS1,and the correlations between genes expression levels and caffeine content were conducted. The results suggested that caffeine content in different tissues presented the order as tender leaves > immature fruits > mature fruits > mature leaves. The caffeine content in immature tissues was significantly higher than that in mature ones（P<0.01）. Gene expression levels of CaXMT1 in the immature tissues was markedly less than that in the mature tissues（P<0.01）,whereas,there was no significant difference among the relative expressions CaDXMT1 and CCS1 in different development stages at the same tissue. Gene expression levels of CaXMT1 and caffeine content in immature fruits demonstrated a significantly negative correlation（R²=0.93,P<0.01）,while,there was no significant correlation between the relative expressions of CaDXMT1,CCS1 and caffeine content in different tissues at different development stages. The biosynthetic pathway of caffeine in coffee（C. arabica）plants is a very complex process,the expression levels of key enzyme genes and their correlations with the caffeine content vary,which might result from their enzymatic properties.

Key words: coffee trees, caffeine, CCS1, CaXMT1, CaDXMT1, genes expression, correlation

LI Fen, LI Li-jiang, XU Dan-ni, CHEN Chun-lin, WANG Wei, LI Mei. Correlation Between Genes Expression Levels of CaXMT1,CaDXMT1,CCS1 in Caffeine Biosynthesis Pathways and Caffeine Content in Coffee arabica[J]. Biotechnology Bulletin, 2019, 35(10): 95-101.

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