Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (10): 25-31.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0092

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Screening of Actinomycetes Against Phytophthora Root Rot of Soybean and Its Growth Promotion and Disease Control

ZHANG Hong-yan1(), GAO qing2, ZHANG Lin-yuan2, LIN Guo-li1, LI Ru-lian1   

  1. 1. College of Life Science and Technology,Lingnan Normal University,Zhanjiang 524048
    2. College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319
  • Received:2020-02-01 Online:2020-10-26 Published:2020-11-02

Abstract:

To screen the strain with good antagonistic effect against the phytophthora root rot of soybean,actinomycete XS1-5 with significant antagonism was isolated from soybean rhizosphere soil by agar block method and mycelia growth assay while taking the Phytophthora sojae of phytophthora root rot pathogen. Strain XS1-5 was identified as Streptomyces albosporeus based on morphological,physiochemical characters as well as molecular methods. The pot experiment was carried out with soybean seeds coated with different spore concentrations of the XS1-5. The results showed the application of the XS1-5 significantly promoted soybean growth,and increased chlorophyll content,root vitality and the protective enzymes in the leaf and root of soybean. When spore concentration was 1.0×10 8 CFU/g,the plant height,root length,fresh weight and dry weight increased by 19.92%、28.26%、87.40% and 34.51%;meanwhile,chlorophyll content(7.53 mg/L)by 50.6% and root vitality(685.41 μg/g·h)by 2.4 folds increased,compared with the control,respectively,as well as the CAT,SOD and POD activity of leaf increased significantly by 158.86%,459.71% and 135.45%. The above results indicate that the antagonistic strain XS1-5 screened in this study has a certain disease-control and growth-promoting effect on soybean.

Key words: phytophthora root rot, actinomycetes, growth promotion, disease control