Optimization of Fermentation Conditions for Production of Feed Containing Functional Components from Distiller’s Grains by Mixed Bacteria Fermentation

doi:10.13560/j.cnki.biotech.bull.1985.2021-0153

Abstract

Abstract:

In order to optimize the fermentation conditions and produce distiller’s grains rich in functional components,the steam explosion pretreatment of distiller’s grains was used as the main raw material,and the strains screened in the early stage was as fermentation strains. The effects of bran addition amount,loading amount,initial pH value,fermentation time,fermentation temperature,and inoculum amount,on the solid-state fermentation of distiller’s grains were studied by taking the crude protein content and nicotinic acid content as the main indexes,and the crude fiber content and acetoin content as the secondary indexes. The results showed that the optimal fermentation conditions were as follows:bran addition 5%,loading 80 g,initial pH 3.70,fermentation time 5 d,fermentation temperature 30℃ and inoculum 10%. Compared with those before optimization,the content of crude protein increased by 1.56%（35.21%）,the content of crude fiber decreased by 33.11%（12.73%）,the content of nicotinic acid increased by 290.32%（1.21 mg/g distiller’s grains）,and the content of acetoin remained unchanged（37.30 mg/g distiller’s grains）.

Key words: mixed fermentation, distiller’s grains, feed, functional component, optimization

FAN En-di, JIANG Meng-ying, FENG Min-xue, CHEN Ye-fu, XIAO Dong-guang, GUO Xue-wu. Optimization of Fermentation Conditions for Production of Feed Containing Functional Components from Distiller’s Grains by Mixed Bacteria Fermentation[J]. Biotechnology Bulletin, 2021, 37(12): 91-103.

Figures/Tables 12

References 38

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水平 Level	因素Factors
水平 Level	A 麸皮添加量 Bran addition amount/%	B 装料量 Loading amount/g	C 初始pH Initial pH
1	5	80	3.70
2	10	100	4.67
3	15	120	5.83

水平 Level	因素Factors
水平 Level	A 麸皮添加量 Bran addition amount/%	B 装料量 Loading amount/g	C 初始pH Initial pH
1	5	80	3.70
2	10	100	4.67
3	15	120	5.83

指标 Indicator	评分标准 Scoring criteria			分数 Score
气味 Smell	无丁酸臭味,有芳香果味或明显的面包香味 With aromatic fruit or obvious bread flavor,but no butyric acid odor			14
	有微弱的丁酸臭味,或较轻的酸味,芳香味弱 With a weak butyric acid odor or a light sour odor,and weak aroma			10
	丁酸味颇重,或有刺鼻的焦糊臭或霉味 With a quite strong butyric acid odor,or a pungent burnt smell or a musty smell			4
	有较强的丁酸臭味或氨味,或几乎无酸味 With a strong butyric acid odor or an ammonia odor,or almost no acid odor			2
质地 Texture	茎叶结构保持良好 Structure of stem and leaf remains well			4
	茎叶结构保持较差 Structure of stem and leaf remains poorly			2
	茎叶结构保持极差,或有轻度的霉菌污染 Structure of stem and leaf remains extremely poor,or there is mild mold contamination			1
	茎叶腐烂或污染严重 Stems and leaves are rotted or heavily polluted			0
色泽 Tinct	与原料相似,烘干后呈淡褐色 Similar to the raw material,it is light brown after drying			2
	略有变色呈淡黄色或带褐色 Slightly discolored,light yellow or brown			1
	变色严重,墨绿色或褐色呈黄色,霉味较强 Serious discoloration,dark green or brown,yellow,and with a strong musty smell			0
总分Total score 等级Grade	16-20	10-15	5-9	0-4
总分Total score 等级Grade	优良（1级） Excellent（Level 1）	尚好（2级） Good（Level 2）	中等（3级） Medium（Level 3）	腐败（4级） Corrupt （Level 4）

指标 Indicator	评分标准 Scoring criteria			分数 Score
气味 Smell	无丁酸臭味,有芳香果味或明显的面包香味 With aromatic fruit or obvious bread flavor,but no butyric acid odor			14
	有微弱的丁酸臭味,或较轻的酸味,芳香味弱 With a weak butyric acid odor or a light sour odor,and weak aroma			10
	丁酸味颇重,或有刺鼻的焦糊臭或霉味 With a quite strong butyric acid odor,or a pungent burnt smell or a musty smell			4
	有较强的丁酸臭味或氨味,或几乎无酸味 With a strong butyric acid odor or an ammonia odor,or almost no acid odor			2
质地 Texture	茎叶结构保持良好 Structure of stem and leaf remains well			4
	茎叶结构保持较差 Structure of stem and leaf remains poorly			2
	茎叶结构保持极差,或有轻度的霉菌污染 Structure of stem and leaf remains extremely poor,or there is mild mold contamination			1
	茎叶腐烂或污染严重 Stems and leaves are rotted or heavily polluted			0
色泽 Tinct	与原料相似,烘干后呈淡褐色 Similar to the raw material,it is light brown after drying			2
	略有变色呈淡黄色或带褐色 Slightly discolored,light yellow or brown			1
	变色严重,墨绿色或褐色呈黄色,霉味较强 Serious discoloration,dark green or brown,yellow,and with a strong musty smell			0
总分Total score 等级Grade	16-20	10-15	5-9	0-4
总分Total score 等级Grade	优良（1级） Excellent（Level 1）	尚好（2级） Good（Level 2）	中等（3级） Medium（Level 3）	腐败（4级） Corrupt （Level 4）

试验号Test No.	A	B	C	试验结果Test results
试验号Test No.	A	B	C	粗蛋白含量 Crude protein content/%	粗纤维含量 Crude fiber content/%	乙偶姻含量 Acetoin content/（mg·g^-1distiller’s grains）	烟酸含量 Nicotinic acid content/（mg·g^-1distiller’s grains）
1	1	1	1	35.21	12.73	37.30	1.21
2	1	2	3	32.10	13.16	15.68	3.55
3	1	3	2	33.62	12.69	29.68	2.14
4	2	1	2	30.56	11.54	28.79	1.46
5	2	2	1	34.66	12.27	29.02	1.88
6	2	3	3	30.70	11.49	3.65	3.10
7	3	1	3	29.19	10.34	11.65	1.17
8	3	2	2	30.31	10.71	0.00	1.23
9	3	3	1	32.09	9.86	31.22	0.84
粗蛋白含量 Crude protein content/%
k1	33.64	31.65	33.99
k2	31.97	32.36	31.50
k3	30.53	32.14	30.66
R	3.11	0.71	3.33
粗纤维含量 Crude fiber content/%
k1	12.86	11.54	11.62
k2	11.77	12.05	11.64
k3	10.30	11.35	11.66
R	2.56	0.70	0.04
乙偶姻含量Acetoin content/（mg·g^-1distiller’s grains）
k1	27.55	25.91	32.51
k2	20.49	14.90	19.49
k3	14.29	21.52	10.33
R	13.26	11.01	22.18
烟酸含量Nicotinic acid content /（mg·g^-1distiller’s grains）
k1	2.30	1.28	1.31
k2	2.15	2.22	1.61
k3	1.08	2.03	2.61
R	1.22	0.94	1.30