Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (4): 230-241.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0704
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Received:
2021-06-02
Online:
2022-04-26
Published:
2022-05-06
Contact:
YU Cun
E-mail:1049286259@qq.com;chifengyucun@163.com
ZHU Jing, YU Cun. Effects of Trichoderma longibrachiatum on Maize Growth,Soil Fertility and Rhizosphere Microorganism[J]. Biotechnology Bulletin, 2022, 38(4): 230-241.
不同处理 Different treatment | 苗高 Height /cm | 根长 Root length/cm | 侧根数 Lateral root number | 干重Dry weight/(g·plant-1) | 鲜重Fresh weight/(g·plant-1) | ||
---|---|---|---|---|---|---|---|
地上Shoot | 地下Root | 地上Shoot | 地下Root | ||||
YJ | 24.20±0.61a | 18.40±0.53a | 8.67±2.08a | 0.11±0.01a | 0.08±0.01a | 1.09±0.40a | 1.37±0.14a |
YF | 23.40±1.73a | 17.93±1.40a | 7.00±2.65a | 0.08±0.03a | 0.05±0.01b | 0.84±0.09a | 0.69±0.33a |
YD | 16.73±1.58b | 10.47±0.15b | 2.67±1.15b | 0.06±0.01b | 0.02±0.01c | 0.54±0.06b | 0.30±0.11b |
Table 1 Effect of T. longissima fertilizer on maize growth
不同处理 Different treatment | 苗高 Height /cm | 根长 Root length/cm | 侧根数 Lateral root number | 干重Dry weight/(g·plant-1) | 鲜重Fresh weight/(g·plant-1) | ||
---|---|---|---|---|---|---|---|
地上Shoot | 地下Root | 地上Shoot | 地下Root | ||||
YJ | 24.20±0.61a | 18.40±0.53a | 8.67±2.08a | 0.11±0.01a | 0.08±0.01a | 1.09±0.40a | 1.37±0.14a |
YF | 23.40±1.73a | 17.93±1.40a | 7.00±2.65a | 0.08±0.03a | 0.05±0.01b | 0.84±0.09a | 0.69±0.33a |
YD | 16.73±1.58b | 10.47±0.15b | 2.67±1.15b | 0.06±0.01b | 0.02±0.01c | 0.54±0.06b | 0.30±0.11b |
Fig.2 Effects of T. longibrachiatum fertilizer on physiology of maize seedlings A:Soluble protein. B:Soluble sugar. C:Chlorophyll. D:Root activity. E:CAT. F:SOD. G:POD shoot. H:POD root
不同处理 Different treatment | 全磷Total phosphorus/(g·kg-1) | 有效磷Available pho-sphorus /(mg·kg-1) | 全钾Total potas-sium/(g·kg-1) | 速效钾Available Pota-ssium/(mg·kg-1) | 全氮Total nitro- gen /(g·kg-1) | 水解氮Hydrolyzed nitrogen /(mg·kg-1) |
---|---|---|---|---|---|---|
YJ | 2.25±0.10a | 44.94±4.67a | 2.70±0.35a | 4.18±0.29a | 14.33±2.65a | 57.87±6.47a |
YF | 2.07±0.18a | 43.62±4.09a | 2.47±0.12ab | 3.98±0.25a | 12.88±1.48a | 54.60±7.89a |
YD | 1.63±1.58b | 29.45±3.84b | 2.08±0.56b | 3.66±0.72b | 7.37±0.58b | 46.67±4.28b |
Table 2 Effects of T. longibrachiatum fertilizer on soil nutrients in the rhizosphere of maize
不同处理 Different treatment | 全磷Total phosphorus/(g·kg-1) | 有效磷Available pho-sphorus /(mg·kg-1) | 全钾Total potas-sium/(g·kg-1) | 速效钾Available Pota-ssium/(mg·kg-1) | 全氮Total nitro- gen /(g·kg-1) | 水解氮Hydrolyzed nitrogen /(mg·kg-1) |
---|---|---|---|---|---|---|
YJ | 2.25±0.10a | 44.94±4.67a | 2.70±0.35a | 4.18±0.29a | 14.33±2.65a | 57.87±6.47a |
YF | 2.07±0.18a | 43.62±4.09a | 2.47±0.12ab | 3.98±0.25a | 12.88±1.48a | 54.60±7.89a |
YD | 1.63±1.58b | 29.45±3.84b | 2.08±0.56b | 3.66±0.72b | 7.37±0.58b | 46.67±4.28b |
不同处理Different treatment | 脲酶Urease/(mg·g-1·d-1) | 蔗糖酶Sucrase/(mg·g-1·d-1) | 磷酸酶Phosphatase/(mg·g-1·d-1) |
---|---|---|---|
YJ | 128.00±2.88a | 19.60±1.09a | 2.45±0.30a |
YF | 99.06±4.99b | 16.09±8.57a | 2.43±0.21a |
YD | 93.40±5.44b | 3.18±0.37b | 2.05±0.66a |
Table 3 Effects of T. longibrachiatum fertilizer on enzyme activities in maize rhizosphere soil
不同处理Different treatment | 脲酶Urease/(mg·g-1·d-1) | 蔗糖酶Sucrase/(mg·g-1·d-1) | 磷酸酶Phosphatase/(mg·g-1·d-1) |
---|---|---|---|
YJ | 128.00±2.88a | 19.60±1.09a | 2.45±0.30a |
YF | 99.06±4.99b | 16.09±8.57a | 2.43±0.21a |
YD | 93.40±5.44b | 3.18±0.37b | 2.05±0.66a |
处理 Treatment | 细菌Bacteria | 真菌Fungi | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
ACE | Chao1 | Simpson | Shannon | ACE | Chao1 | Simpson | Shannon | |||
YJ | 1015.42±82.20b | 1009.43±80.19a | 0.992±0.001a | 8.40±0.15a | 134.14±23.22a | 133.19±22.43a | 0.80±0.12a | 3.46±0.84a | ||
YF | 1317.34±214.63a | 1315.24±224.24a | 0.988±0.010a | 8.58±0.46a | 105.54±26.19a | 104.96±26.27a | 0.76±0.49a | 3.27±0.21a | ||
YD | 1292.47±87.36ab | 1293.86±93.79a | 0.991±0.020a | 8.53±0.20a | 111.37±16.12a | 110.87±16.54a | 0.82±0.35a | 3.46±0.48a |
Table 4 Microbial diversity index of each treatment sample
处理 Treatment | 细菌Bacteria | 真菌Fungi | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
ACE | Chao1 | Simpson | Shannon | ACE | Chao1 | Simpson | Shannon | |||
YJ | 1015.42±82.20b | 1009.43±80.19a | 0.992±0.001a | 8.40±0.15a | 134.14±23.22a | 133.19±22.43a | 0.80±0.12a | 3.46±0.84a | ||
YF | 1317.34±214.63a | 1315.24±224.24a | 0.988±0.010a | 8.58±0.46a | 105.54±26.19a | 104.96±26.27a | 0.76±0.49a | 3.27±0.21a | ||
YD | 1292.47±87.36ab | 1293.86±93.79a | 0.991±0.020a | 8.53±0.20a | 111.37±16.12a | 110.87±16.54a | 0.82±0.35a | 3.46±0.48a |
Fig. 4 Community structure of each sample at phylum level A:Histogram of colony structure at the level of Mycota. B:Histogram of colony structure at the level of bacterial phylum. C:Colony structure heat map at the level of Mycota. D:Colony structure heat map at bacterial phylum level
Fig. 5 Community structure of each sample at genus level A:Histogram of colony structure at the level of fungi. B:Histogram of colony structure at the bacterial genus level. C:Colony structure heat map at the fungal genus level. D:Colony structure heat map at the bacterial genus level
Fig. 7 RDA analysis of soil microbial community and soil nutrients in maize rhizosphere A:Fungus. B:Bacteria. TK:Total potassium. TN:Total nitrogen. TP:Total phosphorus. AK:Available potassium. AP:Available phosphorus. HN:Hydrolyzed nitrogen
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