Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (3): 149-156.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0847

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Study on Antibacterial Stability of Musca domestica Cecropin-MDC Against Salmonella typhimurium

WANG Zi-yan1(), WANG Jian1, ZHANG Lun1, GUI Shui-qing2, LU Xue-mei1()   

  1. 1. School of Life Science and Biopharmaceutics,Guangdong Key Laboratory of Pharmaceutical Bioactive Substances,Guangdong Pharmaceutical University,Guangzhou 510006
    2. Intensive Care Unit,Shenzhen Second People’s Hospital,the First Affiliated Hospital of Shenzhen University,Shenzhen 518031
  • Received:2021-07-08 Online:2022-03-26 Published:2022-04-06
  • Contact: LU Xue-mei E-mail:694467242@qq.com;luxuemei@gdpu.edu.cn

Abstract:

This work aims to investigate the antibacterial stability of the housefly antimicrobial peptide(Musca domestica cecropin,MDC)against Salmonella typhimurium under different conditions. The Oxford cup method was used to determine the influences of pH,temperature,light,protease,metal ions,surfactants and organic reagents on the antibacterial activity(bacteriostatic ring size)of MDC against S. typhimurium. The results showed that the activity of MDC decreased slightly under acidic condition(remained 85% of that under the normal condition),but not changed under alkaline condition with a good stability. Under low or high temperature treatment,no obvious difference was observed in its antibacterial activity. In addition,the activity of MDC still remained stable after exposed to darkness,normal light or ultraviolet for 30 min. MDC was sensitive to trypsin and its activity completely disappeared,but good stability to pepsin. MDC was still stable in the Ca2+ and K+,but it was sensitive to Mg2+. Interestingly,the antibacterial activity of MDC under Fe3+increased compared with the control group(P<0.05). Moreover,the antimicrobial activities of MDC treated with surfactant and organic reagents significantly reduced but still partially preserved. To sum up,MDC can significantly inhibit the growth and reproduction of S. typhimurium,and its activity is almost not affected by the change of pH value/temperature/light and different pepsin and metal ions. Ferric ion can synergistically enhance the antibacterial activity of MDC,but Mg2+,surfactant,organic reagent and trypsin hydrolysis caused its antibacterial activity partially or completely lost,respectively.

Key words: Musca domestica cecropin, stability, Salmonella typhimurium, antibacterial activity