Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (7): 205-214.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0248

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Characteristics of Soil Bacterial Community Structure in the Different Developmental Stages of Desert Grassland Caragana korshinskii Kom. Nebkhas

WANG Zi-yin1,2,3(), LIU Bing-ru1,2,3(), LI Zi-hao1,2,3, ZHAO Xiao-yu1,2,3   

  1. 1. College of Biological Science and Engineering,North Minzu University,Yinchuan 750021
    2. Key Laboratory of Ecological Protection of Agro-pastoral Ecotones in the Yellow River Basin,National Ethnic Affairs Commission of the People’s Republic of China,Yinchuan 750021
    3. Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments,Yinchuan 750021
  • Received:2022-03-01 Online:2022-07-26 Published:2022-08-09
  • Contact: LIU Bing-ru E-mail:846334252@qq.com;bingru.liu@163.com

Abstract:

To understand the soil bacterial community structure and diversity characteristics and the influencing factors at different developmental stages of Caragana korshinskii Kom. nebkhas in arid and semi-arid desert grasslands,C. korshinskii Kom. nebkhas in Ningxia desert grasslands at three different developmental stages of growing,mature,and recession were selected. At the same time,high-throughput sequencing technology was applied to analyze their soil bacterial community structure and diversity characteristics,and to investigate the influencing factors of developmental stages and soil physicochemical factors evolution on soil bacterial community structure and function. Finally,PICRUSt method was to predict the functions of soil bacteria at different developmental stages of C. korshinskii Kom. nebkhas. The results showed that the obtained 15 383 operational taxonomic units(OTUs)belonged to 50 phyla,146 classes,364 orders,590 families,and 1 111 genera,with the largest number of bacteria at the growing stage and the dominant phyla being 9 of Actinobacteriota,Proteobacteria,Acidobacteriota,Chloroflexi,Crenarchaeota,and nine others. Proteobacteria,Acidobacteriota,Chloroflexi,and others,accounting for > 90% of the total number of microorganisms,and the dominant phyla were similar to most studies. There were significant differences in soil AP and EC in different development stages of C. korshinskii Kom. nebkhas,showing mature> growing > recession. Other soil nutrients were not significantly different,but the values were also expressed as mature> growing > recession. There was no significant difference in the diversity and abundance of bacterial communities,but the numerical value was the highest in the mature period. AP and SOC were the main factors influencing the diversity of soil bacterial communities in C. korshinskiiKom. nebkhas. Other environmental factors such as EC and pH also significantly affected the richness of multiple bacterial communities. Based on the prediction of 16S rRNA sequences of soil bacterial genomes in different developmental stages of C. korshinskii Kom. nebkhas,it was found that metabolism was the main function of soil bacteria. The above results can enrich the theoretical knowledge of soil microbial diversity in semi-arid desert grasslands and provide a reference for the restoration of C. korshinskii Kom. vegetation.

Key words: desert steppe, Caragana korshinskii Kom., nebkhas, soil bacteria, community structure, functional prediction