Aptamer of Pseudomonas aeruginosa: Acquiring and Application

doi:10.13560/j.cnki.biotech.bull.1985.2023-0336

Abstract

Abstract:

Pseudomonas aeruginosa is an obligate aerobic Gram-negative bacterium, which is the most important microorganism causing opportunistic infections in humans and poses a variety of health threats to humans. Thus, rapid, sensitive and specific detection is crucial for the prevention and treatment of P. aeruginosa infection. At present, there are problems for conventional detection methods，such as time-consuming, high cost and difficult operation, thus more efficient and economical detection methods are needed. Aptamers are oligonucleotides screened from random libraries that can form a variety of complex secondary structures. In recent years, aptamers have attracted widespread attention due to their ease of synthesis and modification, high affinity and strong specificity, and numerous studies of detection, the prevention and treatment of P. aeruginosa based on aptamers have emerged. In this review, the current progresses in screening and tailoring of P. aeruginosa aptamers were summarized, and the application of its aptamers were introduced from two aspects of detection and control of P. aeruginosa, aiming to provide a reference for the development and application of P. aeruginosa aptamer.

Key words: Pseudomonas aeruginosa, aptamer, screening, tailoring, detection, antibacteria, treatment

LIU Xing-yu, LI Jie, ZHU Long-jiao, LI Xiang-yang, XU Wen-tao. Aptamer of Pseudomonas aeruginosa: Acquiring and Application[J]. Biotechnology Bulletin, 2024, 40(1): 186-193.

Figures/Tables 4

References 37

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靶标 Target	筛选方式SELEX type	序列 Sequence（5'-3'）	K_d/（nmol·L^-1）	亲和力检测方式 Affinity detection method	长度Length/nt	参考文献Reference
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTGT-GTTCGTTTCGTCCCTGCTTCCTTCCTTCCTTC-CTTTCTTG	17.27 ± 5.00	流式细胞术	72	[14]
全菌	Cell-SELEX	ATGCACTCTTCTATCGGTAGTTGAGGGTGCGG	12.5±2.4	流式细胞术	32	[15]
外毒素A	Decoy-SELEX	TGTACCGTCTGAGCGATTCGTACCATAGGGTGCTTTTCAAGGCCACACGTTAGTGTAAGCCAGTCAGTGTTAAGGAGTGC	4 200-4 500	表面等离子体共振	80	[16]
脂多糖	Capture-SELEX	CCTTCTAACAGAATGTTGTTAGATAGC	46.2±9.5	等热滴定量热	27	[17]
全菌	Cell-SELEX	GGGAGGAC^FGAU^FGC^FGGGAAC^FU^FGAAGAGU^FU^FU^FGAU^FC^FAU^FGGC^FU^FC^FAGAU^FU^FGAAC^FGC^FU^FGGC^FGGC^FAGAC^FGAC^FUC^FGC^FGC^FGA	—	—	73	[18]
全菌	WB-SELEX	TGGACCTTGCGATTGACAGCAGACATGAGTCAGGACGTGACCGCTGGACCTTGCGATTGACAGCAGACATGAGTCAGGAC	15.16±3.62	流式细胞术	80	[19]
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG	14.55	地高辛标记适配体	60	[20]
丁酰基-高丝氨酸	Structure-switching SELEX	CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGTTTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT	28.47	流式细胞术	99	[21]

靶标 Target	筛选方式SELEX type	序列 Sequence（5'-3'）	K_d/（nmol·L^-1）	亲和力检测方式 Affinity detection method	长度Length/nt	参考文献Reference
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTGT-GTTCGTTTCGTCCCTGCTTCCTTCCTTCCTTC-CTTTCTTG	17.27 ± 5.00	流式细胞术	72	[14]
全菌	Cell-SELEX	ATGCACTCTTCTATCGGTAGTTGAGGGTGCGG	12.5±2.4	流式细胞术	32	[15]
外毒素A	Decoy-SELEX	TGTACCGTCTGAGCGATTCGTACCATAGGGTGCTTTTCAAGGCCACACGTTAGTGTAAGCCAGTCAGTGTTAAGGAGTGC	4 200-4 500	表面等离子体共振	80	[16]
脂多糖	Capture-SELEX	CCTTCTAACAGAATGTTGTTAGATAGC	46.2±9.5	等热滴定量热	27	[17]
全菌	Cell-SELEX	GGGAGGAC^FGAU^FGC^FGGGAAC^FU^FGAAGAGU^FU^FU^FGAU^FC^FAU^FGGC^FU^FC^FAGAU^FU^FGAAC^FGC^FU^FGGC^FGGC^FAGAC^FGAC^FUC^FGC^FGC^FGA	—	—	73	[18]
全菌	WB-SELEX	TGGACCTTGCGATTGACAGCAGACATGAGTCAGGACGTGACCGCTGGACCTTGCGATTGACAGCAGACATGAGTCAGGAC	15.16±3.62	流式细胞术	80	[19]
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG	14.55	地高辛标记适配体	60	[20]
丁酰基-高丝氨酸	Structure-switching SELEX	CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGTTTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT	28.47	流式细胞术	99	[21]