Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (4): 287-296.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0935
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TENG Wen-long(), WU Yong-na, WANG De-fu, NIU Yan-bing()
Received:
2023-10-06
Online:
2024-04-26
Published:
2024-04-30
Contact:
NIU Yan-bing
E-mail:1294171341@qq.com;niuyanbingbest@163.com
TENG Wen-long, WU Yong-na, WANG De-fu, NIU Yan-bing. Effect of Forsythia suspensa Leaves Tea on HCC Proliferation and Migration Function and Its Mechanism of Action[J]. Biotechnology Bulletin, 2024, 40(4): 287-296.
引物名称 Primer name | 序列 Sequence(5'-3') |
---|---|
GAPDH-F | ATGTTGCAACCGGGAAGGAA |
GAPDH-R | AGGAAAAGCATCACCCGGAG |
Ki-67-F | CTGACCCTGATGAGAGTGAGGGA |
Ki-67-R | GGACAGGTGGAGTGTGCATTA |
Erk-F | TCCTTTGAGCCGTTTGGAGG |
Erk-R | AGTACATACTGCCGCAGGTC |
PI3K-F | GGACCCGATGCGGTTAGAG |
PI3K-R | ATCAAGTGGATGCCCCACAG |
mTOR-F | GCCGCGCGAATATTAAAGGA |
mTOR-R | CTGGTTTCCTCATTCCGGCT |
Table 1 Primer sequences for RT-PCR
引物名称 Primer name | 序列 Sequence(5'-3') |
---|---|
GAPDH-F | ATGTTGCAACCGGGAAGGAA |
GAPDH-R | AGGAAAAGCATCACCCGGAG |
Ki-67-F | CTGACCCTGATGAGAGTGAGGGA |
Ki-67-R | GGACAGGTGGAGTGTGCATTA |
Erk-F | TCCTTTGAGCCGTTTGGAGG |
Erk-R | AGTACATACTGCCGCAGGTC |
PI3K-F | GGACCCGATGCGGTTAGAG |
PI3K-R | ATCAAGTGGATGCCCCACAG |
mTOR-F | GCCGCGCGAATATTAAAGGA |
mTOR-R | CTGGTTTCCTCATTCCGGCT |
Fig. 1 Comparison of phillyrin content between F. suspen-sa green tea and F. suspensa black tea A: Phillyrin standard; B: Forsythia suspensa green tea sample; C: F. suspensa black tea sample
Fig. 2 Comparison of F. glycoside A content between F. suspensa green tea and F. suspensa black tea A: Forsythia glycoside A standard; B: F. suspensa green tea sample; C: F. suspensa black tea sample
Fig. 3 Effect of crude extract of F. suspensa leaves tea on hepatocellular carcinoma line LM3 A: Effect of F. suspensa green tea on the proliferation of HCC LM3. B: Effect of F. suspensa black tea on the proliferation of HCC LM3. C: Control group. LQLCT-L: Low concentration of crude extract from F. suspensa green tea. LQLCT-M: Medium concentration in the crude extract of F. suspensa green tea. LQLCT-H: High concentration of crude extract from F. suspensa green tea. LQHCT-L: Low concentration of crude extract from F. suspensa black tea. LQHCT-M: Medium concentration in the crude extract of F. suspensa black tea. LQHCT-H: High concentration of crude extract from F. suspensa black tea; Comparison with control group, *P<0.05, **P<0.01, ***P<0.001, ****P<0.000 1. The same below
Fig. 4 Effects of phillyrin and F. glycoside A on hepatocellular carcinoma line LM3 A: Effect of Phillyrin on the proliferation of HCC LM3. B: Effect of Forsythia glycoside A on the proliferation of HCC LM3. C: Control group. LQG-L: Low concentration group of Phillyrin. LQG-M: Medium concentration group of Phillyrin. LQG-H: High concentration group of Phillyrin. LQZGA-L: Low concentration group of Forsythia glycoside A. LQZGA-M: Medium concentration group of Forsythia glycoside A. LQZGA-H: High concentration group of Forsythia glycoside A
Fig. 5 Effect of crude extract of F. suspensa green tea (A) and black tea (B), phillyrin (C), and Forsythia glycoside A (D) on the migration ability of LM3 cells
Fig. 6 Effects of crude extract of F. suspensa leaves tea on the proliferation and migration of HCC LM3 A: Effects of F. suspensa green tea and F. suspensa black tea on the expression of Ki-67 in HCC LM3. B: Effects of F. suspensa green tea and F. suspensa black tea on the expression of Erk in HCC LM3. C: Effects of F. suspensa green tea and F. suspensa black tea on the expression of PI3K in HCC LM3. D: Effects of F. suspensa green tea and F. suspensa black tea on the expression of mTOR in HCC LM3. CK: Control group. LQLCT: F. suspensa green tea crude extract group; LQHCT: F. suspensa black tea crude extract group
Fig. 7 Effect of phillyrin and Forsythia glycoside A on the proliferation and migration of HCC LM3 A: Effects of phillyrin and Forsythia glycoside A on the expression of Ki-67 in HCC LM3. B: Effects of phillyrin and Forsythia glycoside A on the expression of Erk in HCC LM3. C: Effects of phillyrin and Forsythia glycoside A on the expression of PI3K in HCC LM3. D: Effects of phillyrin and Forsythia glycoside A on the expression of mTOR in HCC LM3. CK: Control group. LQG: Phillyrin group; LQZGA: Forsythia glycoside A group
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