Function Analysis of Up-regulated Gene(URG11)Based on Its Bioinformatics

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.032

Abstract

Abstract: This work was to obtain differentially-expressed genes with hepatoma cell HepG(HepG2-X)Y of stable transfected HBx and non-transfected hepatoma cell HepG₂using gene chip technology. The preliminary bioinformatic analysis demonstrated that the gene of the protein encoded 673 amino acids with a predicted molecular weight of 17.06 kD and pI 4.83. URG11 were mainly localized in the nuclear and played the role in transcription regulation, growth factor and signal transducer, and it showed 76%-97% identity with the others reported 12 species, as well as was consistent with the evolutionary relationship between species.

Key words: HBV-related hepatocellular carcinoma, bioinformatics, URG11, HBx

YAO Yang, SU Jie, LIU Kai-ge, Xu Rui. Function Analysis of Up-regulated Gene(URG11)Based on Its Bioinformatics[J]. Biotechnology Bulletin, 2016, 32(3): 203-208.

References

[1]Wang C, Yang W, Yan HX, et al. Hepatitis B virus X(HBx)indu-ces tumorigenicity of hepatic progenitor cells in 3, 5-diethoxycarbo-nyl-1, 4-dihydrocollidine-treated HBx transgenic mice[J]Hepato-logy, 2012, 55(1)：108-120.
[2]Yuan W, Huang T, Yu J, et al. Comparative analysis of viral protein interaction networks in Hepatitis B Virus and Hepatitis C Virus infected HCC[J]. Biochim Biophys Acta, 2013, 6(2)：512-516.
[3]Gong P, Zhang X, Zhang J, et al. Comparative analysis of viral protein interaction networks in Hepatitis B Virus and Hepatitis C Virus infected HCC[J]. Front Biosci, 2013, 18：1256-65.
[4]Tang RX, Kong FY, Fan BF, et al. HBx activates FasL and mediates HepG2 cell apoptosis through MLK3-MKK7-JNKs signal module[J]. World Journal of Gastroenterology, 2012, 18(13)：1485-1495.
[5]Holotnakova T^,Tylkova L, Takacova M. Role of the HBx oncoprotein in carbonic anhydrase 9 induction[J]. J Med Virology, 2010, 82(1)：126-129.
[6]Tong GD, Zhang X, Zhou DQ, et al. Efficacy of early treatment on 52 patients with preneoplastic hepatitis B virus-associated hepatocellular carcinoma by compound Phyllanthus urinaria L. [J]. Chin J Integr Med, 2014, 20(4)：263-271.
[7]Yuan K, Lian Z, Sun B, et al. Role of miR-148a in hepatitis B assoc-iated hepatocellular carcinoma[J]. PLoS One, 2012, 7(4)：1-12.
[8]Reno C, Marchuk L, Sciore P, et al. Rapid isolation of total RNA from small samples of hvpocellular dense connective tissues[J]. Bio Techniques, 1997, 22：1082-1086
[9]Altschul SF, Madden TL, Sch?ffer AA, et al. Gapped BLAST and PSI-BLAST：A new generation of protein database search programs[ J ]. Nucleic Acids Res, 1997, 25(17)：3389-3402.
[10] Wilkins MR, Gasteiger E, Bairoch A, et al. Protein identification and analysis tools on the ExPASy server[J] . Methods Mol Biol, 1999, 112：531-52.
[11]王庆浩, 陈爱华, 张伯礼.丹参：一种中药研究的模式生物[J].中医药学报, 2009, 37(4)：1-3.
[12]Chen Y, Yu P, Luo J, Jiang Y. Secreted protein prediction system combining CJ-SPHMM, TMHMM, and PSORT[J]. Mammalian Genome, 2003, 14(12)：859-865
[13]Mukhopadhyay D, Riezman H. Proteasome-independent functions of ubiquitin in endocytosis and signaling[J]. Science, 2007, 315：201-205.
[14]Nielsen H, Engelbrecht J, Brunak S, von Heijne G. A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites[ J ]. International Journal of Neural Systems, 1997, 8(5)：581-599.
[15]Arnold K, Bordoli L, Kopp J, Schwede T. The SWISS-MODEL workspace：a web-based environment for protein structure homology modeling[J]. Bioinformatics, 2006, 22(2)：195-201.
[16]Kiefer F, Arnold K, Kunzli M, et al. The SWISS-MODEL repository and associated resources[J]. Nucleic Acids Research, 2009, 37：D387-D392.
[17]Lian Z, Liu J, Li L, et al. Enhanced cell survival of Hep3B Cell by the hepatitis B x antigen effector, URG11, is associated with upregulation of beta-catenin [J]. Hepatology, 2006, 43(3)：415-424.
[18]Du R, Zhao L, Xia L. Association of URG11 and twist with clinical pathological characteristics and prognosis in patients with IgA nephropathy [J]. Nephrol Dial Transplant, 2013, 28(9)：2268-2276.
[19]Peng W, Zhang J, Liu J. URG11 predicts poor prognosis of pancreatic cancer by enhancing epithelial-mesenchymal transition-driven invasion [J]. Med Oncol, 2014, 31(7)：64.
[20]Fan R Li X, Du W, et al. Adenoviral-mediated RNA interference targeting URG11 inhibits growth of human hepatocellular carcinoma[J]. Int J Cancer, 2011, 128(12)：2980-2993.