Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (4): 179-188.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1069
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LIU Huan-huan1,2(), YANG Li-chun1, LI Huo-gen1()
Received:
2023-11-14
Online:
2024-04-26
Published:
2024-04-30
Contact:
LI Huo-gen
E-mail:lhh91@jsafc.edu.cn;hgli@njfu.edu.cn
LIU Huan-huan, YANG Li-chun, LI Huo-gen. Cloning and Functional Analysis of LtMYB305 in Liriodendron tulipifera[J]. Biotechnology Bulletin, 2024, 40(4): 179-188.
Fig. 1 Anthocyanidin content analysis during floral nectary growth of L. tulipifera * indicates significant difference at P<0.05 level; ** indicates significant difference at P<0.01 level. The same below
蛋白 名称 Protein name | 可信度Reliability | |||
---|---|---|---|---|
叶绿体转运肽Chloroplast transit peptide, cTP | 线粒体靶向肽Mitochondrial targeting peptide, mTP | 分泌通路信号肽Secretory pathway signal peptide, SP | 其他 Other | |
LtMYB305 | 9.40 | 4.90 | 8.00 | 94.70 |
Table 1 Prediction of LtMYB305 protein subcellular localization in L. tulipifera by SignalP-4.0 Server %
蛋白 名称 Protein name | 可信度Reliability | |||
---|---|---|---|---|
叶绿体转运肽Chloroplast transit peptide, cTP | 线粒体靶向肽Mitochondrial targeting peptide, mTP | 分泌通路信号肽Secretory pathway signal peptide, SP | 其他 Other | |
LtMYB305 | 9.40 | 4.90 | 8.00 | 94.70 |
蛋白名称 Protein name | 亚细胞结构Subcellular structure | |||
---|---|---|---|---|
细胞核 Nuclear | 过氧化物酶体Peroxisome | 叶绿体Chloroplast | 液泡 Vacuole | |
MYB305 | 13 | 1 | - | - |
Table 2 Prediction of LtMYB305 protein subcellular localization in L. tulipifera by WOLF PSORT
蛋白名称 Protein name | 亚细胞结构Subcellular structure | |||
---|---|---|---|---|
细胞核 Nuclear | 过氧化物酶体Peroxisome | 叶绿体Chloroplast | 液泡 Vacuole | |
MYB305 | 13 | 1 | - | - |
Fig. 5 Expression analysis of T1 generation of positive 35S::LtMYB305-overexpressed A. thaliana plants A: Semi-quantitative PCR analysis in eight lines of T1 generation positive plants; B: RT-qPCR analysis in eight lines of T1 generation positive plants; C: RT-qPCR analysis in three high-expressed lines of T1 generation positive plants; D: RT-qPCR analysis of eleven genes related to nectary development in three high-expressed lines of T1 generation positive plants
Fig. 6 Screening of positive 35S:: LtMYB305-overexpressed A. thaliana plants A: Detection of positive T2-T3 generation 35S::LtMYB305-overexpressed A. thaliana plants. B: Root observation of wild type and 35S::LtMYB305-overexpressed A. thaliana plants. C: Screening of seedling of wild type and 35S::LtMYB305-overexpressed A. thaliana plants
Fig. 7 Phenotype observation of 35S::LtMYB305-overexpressed A. thaliana A: Floral nectaries of wild type A. thaliana(col)plants. B: Floral nectaries 35S::LtMYB305-overexpressed A. thaliana plants. C: T1-T3 generation of 35S::LtMYB305 -overexpressed A. thaliana plants. LN: Lateral nectary
Fig. 9 SEM of floral nectary of 35S::LtMYB305-overexpressed A. thaliana plants A-D: Floral nectaries of wild type A. thaliana(col)plants. E-L: Floral nectaries of 35S::LtMYB305-overexpressed A. thaliana plants. MN: Medial nectary. LN: Lateral nectary. Sto: Stoma
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