Effect of Cultivation Conditions on the Content of Polysaccharide in Ganoderma tsugae and its Antioxidant Activity

doi:10.13560/j.cnki.biotech.bull.1985.2024-0921

Abstract

Abstract:

【Objective】 The aim of this study is to optimize the cultivation and polysaccharide extraction conditions of Ganoderma tsugae, analyze its antioxidant activity in vitro, and clarify the polysaccharide activity of G. tsugae. 【Method】 The agronomic traits and sugar content of G. tsugae were used as indicators to optimize the cultivation conditions and analyze its antioxidant activity in vitro. Furthermore, response surface design was used to optimize the extraction of G. tsugae crude polysaccharide（GTP）and to investigate its protective effect on H₂O₂ induced HepG2 cells. 【Result】 The fruiting body mass and polysaccharide content are the highest, while the optimal cultivating conditions for G. tsugae is shading rate of 60%, temperature of 30℃, humidity of 80%, and CO₂ concentration of 0.03%. Water extracts from G. tsugae also present promising antioxidant activity in vitro, with the ability to scavenge free radicals and resist superoxide anions. The optimal extraction method for sugar is at an extraction temperature of 82℃, extraction time of 5.6 h, and a liquid-solid ratio of 56 mL/g, reaching 1.90%. 【Conclusion】 The cultivation conditions and polysaccharide extraction process of G. tsugae were clarified, and it was confirmed that it has antioxidant activity in vitro. Its protective effect on H₂O₂-induced HepG2 is related to oxidative stress.

Key words: Ganoderma tsugae, agronomic traits, polysaccharides, extraction process, antioxidant activity

ZHANG Xin, WU Ke-yi, ZHU Si-chen, LI Yu, LI Lan-zhou. Effect of Cultivation Conditions on the Content of Polysaccharide in Ganoderma tsugae and its Antioxidant Activity[J]. Biotechnology Bulletin, 2024, 40(11): 47-57.

Figures/Tables 11

References 31

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因素Factor	水平Level
因素Factor	-1	0	1
A 提取温度Extraction temperature/℃	70	80	90
B 提取时间Extraction time/h	4	5	6
C 液料比Liquid-solid ratio/（mL·g^-1）	40	50	60

因素Factor	水平Level
因素Factor	-1	0	1
A 提取温度Extraction temperature/℃	70	80	90
B 提取时间Extraction time/h	4	5	6
C 液料比Liquid-solid ratio/（mL·g^-1）	40	50	60

组别 Group	栽培条件 Cultivation conditions	子实体质量 Quality/g	菌盖长度 Length/cm	菌盖宽度 Width/cm	生物学效率 Biological efficiency/%
1	遮阴率0%	89.85±14.64 a	14.10±1.98 a	7.95±1.55 a	8.99±1.46 a
2	遮阴率90%	94.26±11.16 ab	15.44±2.36 a	8.82±1.84 a	9.43±1.11 ab
3	温度25℃	84.70±10.27 a	12.80±2.73 b	8.02±1.75 a	8.47±1.03 a
4	温度35℃	110.83±14.42 c	15.27±1.91 a	8.74±2.02 a	10.10±1.44 b
5	湿度70%	97.70±13.78 ab	12.78±2.28 b	7.73±1.46 a	9.77±1.38 ab
6	湿度90%	104.83±13.21 b	12.27±2.73 b	9.08±2.38 a	10.48±1.32 b
7	CO₂浓度0.02%	112.41±14.32 c	15.42±2.02 a	9.04±1.62 b	11.24±1.43 c
8	CO₂浓度0.04%	80.57±11.86 a	11.78±2.23 c	7.07±1.45 c	8.06±1.19 a
9	遮阴率60%，温度30℃，湿度80%，CO₂浓度0.03%	112.10±15.52 c	16.20±2.29 d	8.29±1.57 b	11.21±1.55 c

组别 Group	栽培条件 Cultivation conditions	子实体质量 Quality/g	菌盖长度 Length/cm	菌盖宽度 Width/cm	生物学效率 Biological efficiency/%
1	遮阴率0%	89.85±14.64 a	14.10±1.98 a	7.95±1.55 a	8.99±1.46 a
2	遮阴率90%	94.26±11.16 ab	15.44±2.36 a	8.82±1.84 a	9.43±1.11 ab
3	温度25℃	84.70±10.27 a	12.80±2.73 b	8.02±1.75 a	8.47±1.03 a
4	温度35℃	110.83±14.42 c	15.27±1.91 a	8.74±2.02 a	10.10±1.44 b
5	湿度70%	97.70±13.78 ab	12.78±2.28 b	7.73±1.46 a	9.77±1.38 ab
6	湿度90%	104.83±13.21 b	12.27±2.73 b	9.08±2.38 a	10.48±1.32 b
7	CO₂浓度0.02%	112.41±14.32 c	15.42±2.02 a	9.04±1.62 b	11.24±1.43 c
8	CO₂浓度0.04%	80.57±11.86 a	11.78±2.23 c	7.07±1.45 c	8.06±1.19 a
9	遮阴率60%，温度30℃，湿度80%，CO₂浓度0.03%	112.10±15.52 c	16.20±2.29 d	8.29±1.57 b	11.21±1.55 c

来源Source	平方和Sum of squares	自由度Df.	均方Mean square	F	P	显著性Significance
A	0.0091	1	0.0091	8.53	0.0223	*
B	0.0153	1	0.0153	14.34	0.0068	**
C	0.0085	1	0.0085	7.91	0.0260	*
AB	0.0042	1	0.0042	3.96	0.0870
AC	0.0081	1	0.0081	7.59	0.0283	*
BC	0.0009	1	0.0009	0.84	0.3891
A²	0.2605	1	0.2605	243.98	< 0.0001	* *
B²	0.0995	1	0.0995	93.21	< 0.0001	* *
C²	0.0041	1	0.0041	3.85	0.0905
模型Model	0.4363	9	0.0485	45.40	< 0.0001	Significant
残差Residual	0.0075	7	0.0011
失拟项Lack of Fit	0.0041	3	0.0014	1.60	0.3229	Not significant
纯误差Pure Error	0.0034	4	0.0009
总离差Cor Total	0.4438	16
R² = 0.9832	Adj.R² = 0.9615