Hetero-overexpression of Fragaria vescaFveBBX32 Negatively Regulates the Chlorophyll Content and Flowering Time in Arabidopsis thaliana

doi:10.13560/j.cnki.biotech.bull.1985.2025-0574

Abstract

Abstract:

Objective This study aims to clarify the function of transcription factor FveBBX32 in regulating chlorophyll accumulation and flowering time, providing theoretical basis and candidate gene resources for flowering period regulation and cultivation of high-efficiency strawberry (Fragaria×ananassa Duch.) varieties. Method The Fragaria vesca 'Ruegen' cDNA was used as template to clone FveBBX32 gene for bioinformatics analysis and tissue expression analysis. Transgenic Arabidopsis plants were obtained by Agrobacterium infection, the leaf color and flowering phenotype of the transgenic material were observed and recorded, and the expressions of related genes in chlorophyll and flowering pathways were detected by RT-qPCR. Result The CDS length of FveBBX32 genewas 795 bp, encoding 264 amino acids, the protein was hydrophilic with a relative molecular weight of 28 638.63 Da. It contained one B-box domain, belonging to subgroup V of the BBX family, showing close phylogenetic relationship with Rosa chinensis. FveBBX32 was expressed in the roots, stems, leaves, flowers, and fruits of F. vesca, with higher expressions in flowers and leaves. The overexpression of FveBBX32 in Arabidopsis thaliana resulted in yellowing of leaf color and delayed flowering time. The expressions of chlorophyll biosynthesis-related genes AtGUN4, AtCHLH, AtCHL27, and flowering-related genes AtFT, AtSOC1, and AtFUL were all reduced to varying degrees in transgenic lines. Conclusion FveBBX32 is confirmed as a negative regulatory transcription factor of chlorophyll accumulation and flowering time in plants.

Key words: strawberry, BBX32, chlorophyll, flowering, transgenic

MIAO Bai-ling, WANG Yuan-yuan, HOU Wei-wei, ZHANG Yi-ru, CHEN Juan-juan, LI Liang-jie, ZHANG He, ZHU Qing-song, DONG Xiang-xiang. Hetero-overexpression of Fragaria vescaFveBBX32 Negatively Regulates the Chlorophyll Content and Flowering Time in Arabidopsis thaliana[J]. Biotechnology Bulletin, 2025, 41(11): 293-300.

Figures/Tables 9

References 31

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