Construction of Angelica pubescens Core Germplasm and Its Association Analysis of Active Ingredients

doi:10.13560/j.cnki.biotech.bull.1985.2025-0582

Abstract

Abstract:

Objective The genetic diversity of Angelica pubescens germplasm resources was analyzed, a core collection was constructed, and molecular markers associated with the contents of active ingredients were screened, so as to lay the foundation for the breeding of A. pubescens varieties. Method A differential analysis of the contents of active ingredients in 96 germplasms of A. pubescens was conducted, and 8 ISSR core primers was used to analyze the genetic structure and core collection construction. Talssel software was used to perform association analysis between ISSR markers and active ingredients. Result The variation in the phenotype and the content of active ingredients of A. pubescens is rich, and the population structure analysis can divide the materials into 3 subgroups. Gen Al Ex software analysis shows that the genetic diversity of the three subgroups of A. pubescens is relatively high, and the variation occurs mainly within the subgroups. By using a sampling ratio ranging from 15.6% to 58.3%, we constructed a core collection of 37 samples representing the original population. The association analyses of FarmCPU, GLM, and MLM models indicate that the content of CBD is significantly positively correlated with 3 markers (UBC 812, UBC 816, UBC 819) (P<0.05); CBT content is significantly correlated with UBC 816, UBC 817; IPT content is extremely significantly positively correlated with UBC 819 (P<0.01); OST content is significantly correlated with UBC 819 (P<0.05); UMB content is positively correlated with UBC 810, UBC 812 markers. OHN content is only found to be significantly correlated with UBC 816 in the GLM model. Conclusion The germplasms of A. pubescens can be divided into 3 subgroups, and the constructed core collection of 37 samples can represent the genetic diversity level of the original population, and 6 ISSR molecular markers associated with the content of active ingredients are screened.

Key words: Angelica pubescens, coumarin, molecular marker, core germplasm, population structure, association analysis

JIANG Xiao-gang, YU Kai-di, GUO Xiao-liang. Construction of Angelica pubescens Core Germplasm and Its Association Analysis of Active Ingredients[J]. Biotechnology Bulletin, 2025, 41(11): 236-246.

Figures/Tables 14

References 25

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性状 Traits	极小值 Minimal value	极大值 Maximum value	平均值 Mean value	中位数 Median value	标准差 Standard deviation	变异系数 Coefficient of variation （%）
蛇床子素OST	0.002	0.503	0.147	0.140	0.104	70.74
二氢欧山芹醇当归酸酯 CBD	0.021	0.814	0.278	0.242	0.166	59.71
异欧前胡素 IPT	0.000	0.139	0.012	0.010	0.014	116.67
二氢欧山芹醇 CBT	0.002	0.026	0.011	0.011	0.004	36.36
欧前胡素酚 OHN	0.000	0.436	0.138	0.133	0.068	49.28
伞形酮UMB	0.000	0.209	0.063	0.048	0.045	71.43
茎显色程度 JXS	1	4	3.228	3	0.782	24.22
花药显色程度 HYXS	2	3	2.179	3	0.384	17.62
茎生叶叶鞘茸毛丰富程度 YQRM	1	3	2.582	3	0.594	23.00
茎生叶叶鞘茸毛颜色 RMYS	1	3	1.363	1	0.620	45.49
叶鞘颜色YQYS	1	2	1.145	1	0.352	30.74

性状 Traits	极小值 Minimal value	极大值 Maximum value	平均值 Mean value	中位数 Median value	标准差 Standard deviation	变异系数 Coefficient of variation （%）
蛇床子素OST	0.002	0.503	0.147	0.140	0.104	70.74
二氢欧山芹醇当归酸酯 CBD	0.021	0.814	0.278	0.242	0.166	59.71
异欧前胡素 IPT	0.000	0.139	0.012	0.010	0.014	116.67
二氢欧山芹醇 CBT	0.002	0.026	0.011	0.011	0.004	36.36
欧前胡素酚 OHN	0.000	0.436	0.138	0.133	0.068	49.28
伞形酮UMB	0.000	0.209	0.063	0.048	0.045	71.43
茎显色程度 JXS	1	4	3.228	3	0.782	24.22
花药显色程度 HYXS	2	3	2.179	3	0.384	17.62
茎生叶叶鞘茸毛丰富程度 YQRM	1	3	2.582	3	0.594	23.00
茎生叶叶鞘茸毛颜色 RMYS	1	3	1.363	1	0.620	45.49
叶鞘颜色YQYS	1	2	1.145	1	0.352	30.74

性状 Traits	二氢欧山芹醇CBT	蛇床子素OST	异欧前胡素IPT	二氢欧山芹醇当归酸酯CBD	欧前胡素酚OHN	伞形酮UMB	茎显色程度JXS	花药显色程度HYXS	茎生叶叶鞘茸毛丰富程度YQRM	茎生叶叶鞘茸毛颜色RMYS	叶鞘颜色YQYS
二氢欧山芹醇CBT		0.074	-0.213	-0.279	0.369**	0.255*	0.051	-0.003	0.250*	-0.093	-0.070
蛇床子素OST	0.074		0.007	-0.185	0.023	0.156	0.089	0.013	0.126	-0.236	0.084
异欧前胡素IPT	-0.213	0.007		-0.118	-0.086	-0.056	-0.127	0.130	-0.065	-0.080	-0.070
二氢欧山芹醇当归酸酯CBD	-0.279	-0.185	-0.118		-0.047	-0.198	-0.176	0.123	-0.214	0.104	0.054
欧前胡素酚OHN	0.369**	0.023	-0.086	-0.047		0.390**	-0.076	0.110	0.246*	-0.129	-0.159
伞形酮UMB	0.255*	0.156	-0.056	-0.198	0.390**		-0.059	0.035	0.179	-0.174	-0.038
茎显色程度JXS	0.051	0.089	-0.127	-0.176	-0.076	-0.059		-0.050	0.150	0.435**	0.202
花药显色程度HYXS	-0.003	0.013	0.130	0.123	0.110	0.035	-0.050		0.108	-0.072	0.152
茎生叶叶鞘茸毛丰富程度YQRM	0.250*	0.126	-0.065	-0.214	0.246*	0.179	0.150	0.108		-0.213	-0.321
茎生叶叶鞘茸毛颜色RMYS	-0.093	-0.236	-0.080	0.104	-0.129	-0.174	0.435**	-0.072	-0.213		0.609**
叶鞘颜色YQYS	-0.070	0.084	-0.070	0.054	-0.159	-0.038	0.202	0.152	-0.321	0.609**

性状 Traits	二氢欧山芹醇CBT	蛇床子素OST	异欧前胡素IPT	二氢欧山芹醇当归酸酯CBD	欧前胡素酚OHN	伞形酮UMB	茎显色程度JXS	花药显色程度HYXS	茎生叶叶鞘茸毛丰富程度YQRM	茎生叶叶鞘茸毛颜色RMYS	叶鞘颜色YQYS
二氢欧山芹醇CBT		0.074	-0.213	-0.279	0.369**	0.255*	0.051	-0.003	0.250*	-0.093	-0.070
蛇床子素OST	0.074		0.007	-0.185	0.023	0.156	0.089	0.013	0.126	-0.236	0.084
异欧前胡素IPT	-0.213	0.007		-0.118	-0.086	-0.056	-0.127	0.130	-0.065	-0.080	-0.070
二氢欧山芹醇当归酸酯CBD	-0.279	-0.185	-0.118		-0.047	-0.198	-0.176	0.123	-0.214	0.104	0.054
欧前胡素酚OHN	0.369**	0.023	-0.086	-0.047		0.390**	-0.076	0.110	0.246*	-0.129	-0.159
伞形酮UMB	0.255*	0.156	-0.056	-0.198	0.390**		-0.059	0.035	0.179	-0.174	-0.038
茎显色程度JXS	0.051	0.089	-0.127	-0.176	-0.076	-0.059		-0.050	0.150	0.435**	0.202
花药显色程度HYXS	-0.003	0.013	0.130	0.123	0.110	0.035	-0.050		0.108	-0.072	0.152
茎生叶叶鞘茸毛丰富程度YQRM	0.250*	0.126	-0.065	-0.214	0.246*	0.179	0.150	0.108		-0.213	-0.321
茎生叶叶鞘茸毛颜色RMYS	-0.093	-0.236	-0.080	0.104	-0.129	-0.174	0.435**	-0.072	-0.213		0.609**
叶鞘颜色YQYS	-0.070	0.084	-0.070	0.054	-0.159	-0.038	0.202	0.152	-0.321	0.609**

种质来源 Germplasm source	观测等位基因数Na	有效等位基因数Ne	Nei’s基因多样性指数H	Shannon’s信息指数I	遗传分化系数Gst	基因流Nm
建始 Jianshi	1.883 3	1.490 0	0.293 8	0.445 2	0.112 6	3.939 4
巴东 Badong	1.983 3	1.494 1	0.298 5	0.457 2
五峰 Wufeng	1.883 3	1.520 9	0.304 3	0.456 4
均值 Mean value	1.916 6	1.500 7	0.298 9	0.452 9	-	-