Genome-wide Identification of Panax notoginseng Lipoxygenases Coupled in Response to Methyl-jasmonate and Wounding

doi:10.13560/j.cnki.biotech.bull.1985.2025-0726

Abstract

Abstract:

Objective To identify the Panax notoginsengLOX gene family and analyze its expression characteristics, thereby providing a basis for further investigation into the biological functions of the PnLOX genes. Method Bioinformatics methods were used to identify P. notoginseng LOX gene family and analyze its the phylogenetic relationship, gene structure characteristics, cis-elements and expression patterns of different plant tissues, MeJA treatment and wounding stress. Result Ten putative PnLOX genes were identified and formed three subgroups (9S-LOX, SG I 13S-LOX and SG II 13S-LOX ) according to phylogenetic tree and LOX substrate specificity. It was observed that LOX genes with similar evolutionary relationships had similar gene structures and conserved motifs. The PnLOX genes was located on six chromosomes and expanded mainly via segmental duplication (SD) and tandem duplication (TD). Subcellular localization prediction revealed that, except for PnLOX1/2/9 from SG I 13S-LOX subgroup, which localized to the chloroplast, all other PnLOX localized to the cytoplasm. Tissue expression analysis indicated that the PnLOX genes were differentially expressed in different tissues. The PnLOX1 and PnLOX2 genes were rapidly activated after MeJA treatment and wounding. Conclusion Ten putative PnLOX genes are identified from the P. notoginseng genome, among which the PnLOX1 and PnLOX2 genes may play an essential role in JA biosynthesis and JA-mediated damage repair in P. notoginseng.

Key words: Panax notoginseng, lipoxygenases (LOX), gene family, wounding, jasmonic acid (JA)

LI Jian-bin, HOU Jia-e, LI Lei-lin, AI Ming-tao, LIU Tian-tai, CUI Xiu-ming, YANG Qian. Genome-wide Identification of Panax notoginseng Lipoxygenases Coupled in Response to Methyl-jasmonate and Wounding[J]. Biotechnology Bulletin, 2026, 42(1): 218-229.

Figures/Tables 8

Table 1 Primer sequences for RT-qPCR

基因名 Gene name	正向引物 Forward primer （5′-3′）	反向引物 Reverse primer （5′-3′）	产物大小 Product （bp）
PnACTIN2	TCCAAGGGTGAATATGATGAATCG	AACCTCTCCAAAGAGAATTTCTGAGT	199
PnLOX1	TAGAGGGCCTTCCTGCTGAT	GCTTGGAGCTCCCTGTCATT	208
PnLOX2	CCCAATGTGGTCAGCAGGAT	TGGGCCATATATCTCCGGGT	207
PnLOX3	GAGGGATGGCAGTTGAGGAC	CATGGCCCTTTTCTCGGACT	210
PnLOX4	CACTCCAGCTGAAGAGGGTG	GCGGAAGTGAGGTTGGAGAA	199
PnLOX5	CCTTTATCTCCGGCGATGCT	AACCTTGGTGGCCTTATCGG	158
PnLOX6	ACCGACAACTAGGTGCGATG	CTGCTGGCAATCCTTCCTGA	210
PnLOX7	CCTTGACCCTGTGAGCTACG	GTTTTCCTTTTCCCGGTGGC	269
PnLOX8	GCAGGTGGAAGCGTTGAAAG	AACTTCAGGCCATAGGGTGC	167
PnLOX9	AAGCAGGGGTCAGTAACAGC	AGATCCCCTGACCAATCCCA	244
PnLOX10	TCGCCTCCATGAGTTTCCAC	AGGTAGAGGAGTGTGCGACT	234

Fig. 1 Phylogenetic tree of LOX proteins of P. notoginseng, A. thaliana, and Oryza sativaDifferent species are indicated in different shapes and colors. The various colors indicate the different subgroups

Fig. 2 Phylogenetic relationship, conserved motifs, conserved domain and gene structure of the PnLOXsA: Phylogenetic tree of PnLOXs. B: Conserved motifs of PnLOXs. C: Conserved domain of PnLOXs. D: Gene structure of PnLOXs. D: Amino acid sequence of PnLOXs conserved motifs

Fig. 3 Chromosomal localization and collinearity analysis of PnLOX gene

Fig. 4 Cis-acting elements in the promoter regions of PnLOX genesA: Distribution of PnLOX genes cis-acting elements on the promoter. Different colors indicate the different cis-acting elements. B: The number of cis-acting elements in PnLOX genes. The heatmap in grid and the color columns indicate the numbers of cis-acting elements

Fig. 5 Expression profiles of PnLOXs in different tissueDifferent letters indicate significant differences (P<0.05). The same below

Fig. 6 Expression profiles of PnLOXs under MeJA treatmentA. Transcriptome expression heatmap of PnLOX genes after MeJA treatment. B. RT-qPCR results of 6 PnLOX genes after MeJA treatment

Fig. 7 Expression profiles of PnLOXs in response to wound stressA: Expressions of PnLOX genes at different times after wound stress. B: Un-wounded and wounded leaf of P. notoginseng

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