Transcriptional Regulation Analysis and Prokaryotic Expression ofahpC Gene Putatively-encoding Alkyl Hydroperoxide Reductase inXanthomonas oryzae pv. oryzae

Abstract

Abstract: The ahpC gene involved in degradation of hydrogen peroxide was cloned from the wild-type strain PXO99^A of Xanthomonasoryzae pv. oryzae in this study. The gene putatively encodes the alkyl hydroperoxide reductase AhpC. The sequence of ahpC protein is highlyconserved in different kinds of pathogenic bacteria tested. To find out the structural characteristics of transcription of the gene cluster oxyR-ahpFahpC,reverse transcription PCR（RT-PCR）analysis revealed that ahpC and ahpF gene, the enzyme electron donor gene are co-transcribed inthe same operon and same transcriptional unit. The activity of ahpCp-lacZ was detected, suggesting the ahpC promoter activity was positivelyregulated by transcriptional regulator factor OxyR. In addition, construction of expression vector of ahpC using pET-28a（+）and prokaryoticexpression was performed. The soluble recombinant proteins were expressed, which could be purified and used for further biological functionanalysis.

Key words: Xanthomonas oryzae pv. oryzae, ahpC gene, Transcriptional regulation, Prokaryotic expression

Wang Yanli, Wu Maosen, Tian Fang, Chen Huamin, He Chenyang . Transcriptional Regulation Analysis and Prokaryotic Expression ofahpC Gene Putatively-encoding Alkyl Hydroperoxide Reductase inXanthomonas oryzae pv. oryzae[J]. Biotechnology Bulletin, 2012, 0(12): 114-119.

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