Cloning and Sequence Analysis of HbNTL1 Promoter from Hevea brasiliensis

Abstract

Abstract: Membrane-bound NAC transcription factors(NTLs)are a group of NAC transcription factors with transmembrane motif(TMs)in the C-terminal region, play important roles in plant growth and development, hormone regulations and stress responses. A 1 718 bp5' regulatory sequence of HbNTL1 was cloned from leaf genomic DNA of H. brasiliensis based on the cDNA sequences using Genome Walkingmethod. Sequence analysis showed that it consisted of a typical core promoter region of eukaryotic, and the transcription start site(TSS, A)located 206 bp upstream of the initiation codon ATG. The promoter contains the basic elements ：TATA-box, CAAT-box, and some hormoneresponsive elements as well as and multiple stress-induced elements, such as, ABRE, DOFCOREZM, MYBCORE, MYCCONSENSUSATHSEand W-box. It is suggested that HbNTL1 gene may play important roles in response to various stresses in rubber tree as a stress-related NACtranscription factor.

Key words: Hevea brasiliensis , HbNTL1 promoter , cis-acting element , Stress responsiveness

Kang Guijuan, Li Yu, Zeng Rizhong, Nie Zhiyi. Cloning and Sequence Analysis of HbNTL1 Promoter from Hevea brasiliensis[J]. Biotechnology Bulletin, 2013, 0(1): 83-88.

References

[1] Puranik S, Sahu PP, Srivastava PS, et al. NAC proteins ：regulationand role in stress tolerance[J]. Trends Plant Sci, 2012, 176（6）：369-381.
[2] Nakashima K, Takasaki H, Mizoi J, et al. NAC transcription factorsin plant abiotic stress responses[J]. BBA-Gene Regul Mech,2012, 1819（2）：97-103.
[3] Nuruzzaman M, Manimekalai R, Sharoni AM, et al. Genome-wideanalysis of NAC transcription factor family in rice[J]. Gene, 2010,465（1-2）：30-44.
[4] Le DT, Nishiyama R, Watanabe Y, et al. Genome-wide survey andexpression analysis of the plant-specific NAC transcription factorfamily in soybean during development and dehydration stress[J].DNA Res, 2011, 18（4）：263-276.
[5] Kim SG, Park CM. Membrane-mediated salt stress signaling inflowering time control[J]. Plant Signal Behav, 2007, 2（6）：517-518.
[6] Seo PJ, Kim SG, Park CM. Membrane-bound transcription factors inplants[J]. Trends Plant Sci, 2008, 13（10）：550-556.
[7] Tran LSP, Nishiyama R, Yamaguchi-Shinozaki K, et al. Potentialutilization of NAC transcription factors to enhance abiotic stresstolerance in plants by biotechnological approach[J]. GM Crops,2010, 1（1）：32-39.
[8] Kim SG, Lee SM, Seo PJ, et al. Genome-scale screening andmolecular characterization of membrane-bound transcription factorsin Arabidopsis and rice[J]. Genomics, 2010, 95（1）：56-65.
[9] Shen H, Yin YB, Chen F, et al. A bioinformatic analysis of NACgenes for plant cell wall development in relation to lignocellulosicbioenergy production[J]. Bioenerg Res, 2009, 2 ：217-232.
[10] Puranik S, Bahadur RP, Srivastava PS, et al. Molecular cloningand characterization of a membrane associated NAC family gene,SiNAC from foxtail millet
[Setaria italica（L.）P. Beauv.][J].Mol Biotechnol, 2011, 49 ：138-150.
[11] Tang YM, Liu MY, Gao SQ, et al. Molecular characterization ofnovel TaNAC genes in wheat and overexpression of TaNAC2aconfers drought tolerance in tobacco[J]. Physiol Plant, 2012,生物技术通报 Biotechnology Bulletin 2013年第1期88144（3）：210-224.
[12] Hao YJ, Wei W, Song QX, et al. Soybean NAC transcription factorspromote abiotic stress tolerance and lateral root formation intransgenic plants[J]. Plant J, 2011, 68（2）：302-313.
[13] Distelfeld A, Pearce SP, Avni R, et al. Divergent functions of orthologousNAC transcription factors in wheat and rice[J]. Plant MolBiol, 2012, 78 ：515-524.
[14] Yang SD, Seo PJ, Yoon HK, et al. The Arabidopsis NACtranscription factor VNI2 integrates abscisic acid signals into leafsenescence via the COR/RD genes[J]. Plant Cell, 2011, 23（6）：2155-2168.
[15] Zhong RQ, Lee CH, Ye ZH. Global analysis of direct targets ofsecondary wall NAC master switches in Arabidopsis[J]. MolPlant, 2010, 3（6）：1087-1103.
[16] An ZW, Wang QT, Hu YS, et al. Co-extraction of high-qualityRNA and DNA from rubber tree（Hevea brasiliensis）[J]. Afr JBiotechnol, 2012, 11 ：9308-9314.
[17] 夏可灿, 康桂娟, 黎瑜, 等. 橡胶树胶乳HbPLDα1 基因机器启动子的克隆与生物信息学分析[J]. 热带作物学报, 2012, 1（33）：63-69.
[18] Higo K, Ugawa Y, Iwamoto M, et al. Plant cis-acting regulatoryDNA elements（PLACE）database ：1999 Nucleic[J]. AcidsRes, 1999, 27（1）：297-300.
[19] Yamaguchi-Shinozaki K, Shinozaki K. Organization of cisactingregulatory elements in osmotic-and cold-stress-responsivepromoters[J]. Trends Plant Sci, 2005, 10（2）：88-94.
[20] Abe H, Yamaguchi-Shinozaki K, Urao T, et al. Role of ArabidopsisMYC and MYB homologs in drought-and abscisic acid-regulatedgene expression[J]. The Plant Cell Online, 1997, 9（10）：1859-1868.
[21] Narusaka Y, Nakashima K, Shinwari ZK, et al. Interaction betweentwo cis-acting elements, ABRE and DRE, in ABA-dependentexpression of Arabidopsis rd29A gene in response to dehydrationand high-salinity stresses[J]. Plant J, 2003, 34（2）：137-148.
[22] Plesch G, Ehrhardt T, Mueller-Roeber B. Involvement of TAAAGelements suggests a role for Dof transcription factors in guard cellspecificgene expression[J]. Plant J, 2001, 28（4）：455-464.
[23] Eulgem T, Rushton PJ, Robatzek S, et al. The WRKY superfamilyof plant transcription factors[J]. Trends Plant Sci, 2000, 5（5）：199-205.
[24] Rushton PJ, Somssich IE, Ringler P, et al. WRKY transcriptionfactors[J]. Trends Plant Sci, 2010, 15（5）：247-258.
[25] Han QQ, Zhang JH, Li HX, et al. Identification and expression patternof one stress-responsive NAC gene from Solanum lycopersicum[J]. Mol Biol Rep, 2011, 39 ：1713-1720.
[26] Nakashima K, Tran LSP, Van Nguyen D, et al. Functional analysisof a NAC-type transcription factor OsNAC6 involved in abiotic andbiotic stress-responsive gene expression in rice[J]. Plant J, 2007,51（4）：617-630.
[27] Fang YJ, You J, Xie KB, et al. Systematic sequence analysis andidentification of tissue-specific or stress-responsive genes of NACtranscription factor family in rice[J]. Mol Genet Genomics, 2008,280 ：547-563.
[28] Zheng XN, Chen B, Lu GJ, et al. Overexpression of a NAC transcriptionfactor enhances rice drought and salt tolerance[J]. BiochemBiophys Res Commun, 2009, 379（4）：985-989.