Antioxidant Activity of Solvent Extracts of Xinjiang Propolis and Determination of Quercetin and Chrysin Propolis

Abstract

Abstract: To study the DPPH free radical scavenging activity and anti-lipid peroxidation activity of Kuqa and Yili, and determine the content of the Quercetin and Chrysin in Xinjiang Yili propolis. Different extracts of raw propolis were extracted by four kinds of solvents, taking tea polyphenol as comparison; measure Kuqa and Yili, Xinjiang propolis extracts antioxidant activity in removal of DPPH（1, 1-Diphenyl- 1-picrylhydrazyl）free radicals and antioxidant properties of linoleic acid peroxidation. With 70% ethanol and methanol as solvent to extract Quercetin and Chrysin from Xinjiang Yili propolis, using HPLC（high performance liquid chromatography）to determine the content of the extracted Quercetin and Chrysin, the detection wavelength was 370 nm and 268 nm, respectively. Result showed that propolis different extracts in different concentrations has different scavenging free radical DPPH capacity, in partial experimental groups’ scavenging capacity is even surpassed to same concentration of tea polyphenol group; has antioxidant activity to linoleic acid, in which Yili propolis has higher antiperoxidation activity. The content of Quercetin was the lowest in the without defatted ethanol extracts（2.2950 mg/g）, the highest in the defatted methanol extracts（2.8150 mg/g）；The content of Chrysin was the lowest in the without defatted methanol extracts（60.72 mg/g）, the highest in the defatted methanol extracts（74.37 mg/g）. The Kuqa and Yili propolis has a certain extent of antioxidant capacity, this DPPH method is fast, sensitive and simple, this results showed that the Xinjiang propolis is a natural antioxidant, and waiting for further study and utilization; HPLC analysis showed that Quercetin and Chrysin the active ingredients of propolis samples from Xinjiang Yili were detected.

Key words: Propolis, Antioxidant, DPPH, TBA, HPLC, Quercetin, Chrysin

Buwihelqem Ababekri, Mutallip Amet Aminigul, Mamat Nizam Ehet, Yimit Rahman . Antioxidant Activity of Solvent Extracts of Xinjiang Propolis and Determination of Quercetin and Chrysin Propolis[J]. Biotechnology Bulletin, 2013, 0(2): 163-171.

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