Effect of Silver Nitrate on Agrobacterium Mediated Genetic Transformation of Cassava

Abstract

Abstract: It was to improve the conversion efficiency of cassava genetic transformation. The effect of silver nitrate on the Agrobacterium mediated genetic transformation of cassava was assessed by the maturation of 10-15 days of Southern China cassava SC8 embryoid cotyledon as explants. The results showed that silver nitrate could improve the regeneration frequency and reduce callus formation in SC8. The use of silver nitrate at concentrations between 2 and 8 mg/L increased shoot organogenesis frequency from 58.5% to 87.5% while callus formation decreased from 92% to 15.3% without the Agrobacterium cocultivation ；and shoot organogenesis frequency increased from 55.3% to 85.3% while callus formation decreased from 87.5% to 8.5% with Agrobacterium cocultivation. Meantime, the silver nitrate optimum concentration of adventitious buds induction by the Agrobacterium mediated genetic transformation was higher than that without the Agrobacterium treatment, the former was 8 mg/L, the latter was 6 mg/L. The application of silver nitrate can stimulate cassava SC8 genetic transformation.

Key words: Silver nitrate, Cassava, Genetic transformation, Adventitious buds induction

Guo Yunling, Yin Qi, Kong Hua, Huang Qixing, Zuo Jiao, He Lika, Guo Anping. Effect of Silver Nitrate on Agrobacterium Mediated Genetic Transformation of Cassava[J]. Biotechnology Bulletin, 2013, 0(3): 166-170.

References

[1] 黄毓文, 刘殷勤. 国际热带农业中心的木薯研究[J]. 热带亚热带植物学报, 1995, 3(2):93-100.
[2] 赵巧阳, 赖钟雄. 硝酸银在离体培养和转化中的作用及其机理[J]. 亚热带农业研究, 2008, 4(1):62-66.
[3] 黄玉吉, 赖钟雄, 赵巧阳. 硝酸银在香蕉遗传转化上的作用[J]. 亚热带农业研究, 2008, 4(1):59-62.
[4] 刘娟, 汤浩茹, 王小蓉, 罗娅. 硝酸银在植物离体培养中的应用之研究进展[J]. 农业生物技术科学, 2007, 3(10):400-407.
[5] 王亚琴, 梁承邺. 硝酸银在水稻农杆菌转化中的作用[J]. 湖南大学学报:自然科学版, 2004, 31(2):28-34.
[6] 乔定君, 毛萍, 马欣荣, 杨宏. 甘露醇及AgN03 对根癌农杆菌介导的多年生黑麦草遗传转化的影响[J]. 草业学报, 2011, 20(1):102-110.
[7] 高武军, 王景雪, 卢龙斗, 等. 硝酸银在油菜基因转化中的影响作用[J]. 中国农学通报, 2002, 18(4):43-47.
[8] 刘金华, 王丕武, 武丽敏, 等. 脯氨酸、硝酸银对农杆菌转化大豆的影响[J]. 大豆科学, 2003, 22(1):36-39.
[9] 钟名其, 楼程富, 谈建中, 等. 硝黢银对桑树遗传转化作用[J]. 热带亚热带植物学报, 2002, 10(1):74-76.
[10] Zhang P, Phansiri S, Puonti-Kaerlas J. Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro[J]. Plant Cell, Tissue and Organ Culture, 2001, 67 :47-54.
[11] 姚庆荣, 郭运玲, 孔华, 等. 影响木薯芽器官发生及植株再生的因素[J]. 安徽农业科学, 2010, 38(30):16781-16783.
[12] 张鹏, 傅爱根, 王爱国. AgNO3 在植物离体培养中的作用及可能的机制[J]. 植物生理通讯, 1997, 33(5):376-379.
[13] 张鹏, 凌定厚. 提高菜心离体植株再生频率的研究[J]. 植物学报, 1995, 3(11):902-908.
[14] 王文星, 屈山, 曹成有, 等. 硝酸银对离体培养烟草叶片愈伤组织形成和芽再生及其脯氨酸和丙二醛含量的影响[J]. 植物生理学通讯, 2006, 42(4):668-670.
[15] 杜虹, 庄东红, 黄文华. AgNO3 对大白菜子叶芽再生的促进作用[J]. 热带亚热带植物学报, 2000, 8(2):109-112.
[16] Palmer EE. Enhanced shoot regeneration from Brassica campestrus by silver nitrate [J]. Plant Cell Rep, 1992, 11 :541.
[17] Sharma KK. Bhojwani SS. et al. Factors affecting high frequency differentiation of shoots and roots from cotyledon explants of Brassica jancea(L.)czern [J]. Plant Sci, 1990, 66(2):247-253.