Screening and Primary Identification of an Aerobic Denitrifier Isolate with Salt Tolerance

Abstract

Abstract: Selective enrichment culture method under oxygen conditions was used for screening bacteria,the 4 strains which have capability of aerobic denitrification was isolated from a shrimp pond. A strong denitrifying effect bacteria was selected from them（F1）. The bacterium reduced the concentrations of nitrate nitrogen rapidly from 442.28 mg/L to 58.28 mg/L, nitrite nitrogen from 32.79 mg/L to 0.98 mg/L with 40 h in aerobic conditions. It identified as Halomonas sp. based on its biochemical and morphological characters. Phylogenetic analysis of 16S rDNA sequences indicated that strain F1 was most closely related to Halomonas alimentaria. The denitrification mainly occurs in the logarithmic growth phase.

Key words: Aerobic denitrifier, Denitrification efficiency, Halomonas sp., Salt tolerance

Shi Xiaotong, Li Yanqin, Xing Gongwei, Kang Xianjiang. Screening and Primary Identification of an Aerobic Denitrifier Isolate with Salt Tolerance[J]. Biotechnology Bulletin, 2013, 0(3): 175-180.

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