Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 186-191.

• Research report • Previous Articles     Next Articles

Prokaryotic Expression and Purification of Metastasis Suppressor Gene KISS-1 and NM23-H1

Zuo Fanglei, Feng Xiujuan, Chen Shangwu   

  1. College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083）
  • Received:2012-09-20 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

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Abstract: Metastasis suppressor gene KISS-1 and NM23-H1 were cloned from human placenta and breast cancer cell,share 100% identity with that published in GenBank. NM23-H1 was successfully expressed in E.coli, the major amount of protein was soluble, accounting for more than 50% of total protein, the purified protein has 90% purity ；however, KISS-1 should be fused to the sequence from expression vector then can effectively expressed, the expressed protein was insoluble inclusion body, accounting for more than 70% of total insoluble protein, after purification, KISS-1 may formed into polymeric structures.

Key words: Metastasis suppressor gene, KISS-1, NM23-H1, Expression, Purification