UV-B Radiation Affects Protein Expression of the Wheat’s Leaf

Abstract

Abstract: The reduction in average global ozone layer may cause the enhance of solar ultradiation adiation（UV-B）that reach to the ground surface. We selected the cash crops of winter wheat which grown in northern China to studied the influence of UV-B radiation on plants. Using the method of two-dimensional electrophoresis to analysis changes of the protein in wheat leaves by UV-B radiation. The results showed that wheat leaf protein of the first four days, eight days changes significantly. The two dimensional gel electrophoresis revealed 15 differentially expressed polypeptides, 3 of which were successful identified by mass spectrometry, including Copper/Zinc Superoxide dismutase, Calmodulin, Rubisco large subunit binding protein subunit alpha. The results showed that enhanced UV-B radiation can be regulating the gene encoding protein of wheat leaf to adjust plant growth.

Key words: UV-B radiation, Protein of wheat leaves, Two-dimensional electrophoresis

Qi Tingting, Duan Jiangyan. UV-B Radiation Affects Protein Expression of the Wheat’s Leaf[J]. Biotechnology Bulletin, 2013, 0(3): 53-59.

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