Review on High-level Aspergillus niger Glucose Oxidase Production Engineering Strains

Abstract

Abstract: Glucose oxidase, which can catalyze the oxidation of glucose to glucono-δ-lactone, has been applied in various fields. The traditional production of glucose oxidase using Aspergillus niger as the industrial production microorganism has reached the limit. Nowadays, the main expression systems which have been used in the expression of glucose oxidase are Asprgillus niger, Saccharomyces cerevisiae and Pichia pastoris. Directed protein evolution has recently come into use in the molecular modification of glucose oxidase, which is expected to improve the yield of Aspergillus niger glucose oxidase in industrial production. The progress of high-level glucose oxidase production engineering strain were reviewed.

Key words: Aspergillus niger, Glucose oxidase, Construction of engineering strain, Expression system, Directed protein evolution

Liu Yu, Li Piwu. Review on High-level Aspergillus niger Glucose Oxidase Production Engineering Strains[J]. Biotechnology Bulletin, 2013, 0(7): 12-19.

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