Progress in Aphid-resistant Genes and Transgenic Crop Research

Abstract

Abstract: Aphids damage, commonly and frequently occurring, caused serious losses to agricultural production. In the paper, we reviewed the progress in genetic engineering for aphid resistance, the genes resistant to aphids and their transgenic research, as well as problems existing in aphid resistance at present, and discussed employment of the methods such as cloning of direct toxin genes, site-directed mutagenesis, increasing of expressed regulation elements, lectin proteins as transport carrier to enhance aphid resistance for the aphid-resistant crops breeding program in the future.

Key words: Aphid, Aphid-resistant gene, Genetic engineering, Transgenic crops

Chen Jin, Liu Zhi, Zhu Shengwei. Progress in Aphid-resistant Genes and Transgenic Crop Research[J]. Biotechnology Bulletin, 2014, 0(10): 1-7.

References

[1] 乔格侠. 蚜虫学研究现状与学科发展趋势[J].昆虫学报, 2006, 49（6）：1017-1026.
[2] SA, Bos JI. Effector proteins that modulate plant-insect interactions[J]. Current Opinion in Plant Biology, 2011, 14（4）：422-428.
[3] G, Gradin T, Ahman I, et al. Microarray analysis of the interaction between the aphid Rhopalosiphum padiand host plants reveals both differences and similarities between susceptible and partially resistant barley lines[J]. Molecular Genetics Genomics, 2009, 281（3）：233-248.
[4] JIB, Prince D, Pitino M, et al. A functional genomics approach identifies candidate effectors from the aphid species Myzus persicae（green peach aphid）[J]. PloS Genetics, 2010, 6（11）：1-13.
[5] LC, Linda LW, Timothy DP. Behavior and biology of the tomato psyllid, Bactericerca cockerelli, in response to the Mi-1.2 gene[J]. Entomologia Experimentalis et Applicata, 2006, 121（1）：67-72.
[6] CL, Walling LL, Paine TD. Behavior and biology of the tomato psyllid, Bactericerca cockerelli, in response to the Mi-1.2 gene[J]. Entomologia Experimentalis et Applicata, 2006, 121（1）：67-72.
[7] Y, Hill CB, Carlson SR, et al. Soybean aphid resistance genes in the soybean cultivars Dowling and Jackson map to linkage group M[J]. Molecular Breeding, 2007, 19（1）：25-34.
[8] J, Creasy R, Gao L, et al. Aphid resistance in Medicago truncatula involves antixenos is and phloem-specific, inducible antibiosis, and maps to a single locus flanked by NBS-LRR resistance gene analogs[J]. Plant Physiology Preview, 2005, 137（4）：1445-1455.
[9] T, Pfeiffer F, Kervella J, et al. Inheritance of green peach aphid resistance in the peach cultivar ‘Rubira’[J]. Plant Breeding, 2002, 121（5）：459-461.
[10] A, Neal JW, McCanna I, et al. Cytokinin-mediated insect resistance in Nicotiana plants transformed with the ipt gene[J]. Plant Molecular Biology, 1993, 23（2）：325-335.
[11] 赵德刚. 转IPT基因油菜提高抗蚜能力[J]. 分子植物育种, 2011, 9（3）：343-349.
[12] SK, Atif SM, Khan RH, et al. Protein proteinase inhibitor genes in combat against insects, pests, and pathogens：natural and engineered phytoprotection[J]. Archives of Biochemistry and Biophysics, 2004, 431（1）：154-159.
[13] L, Martinez M, Alfageme F, et al. A barley cysteine-proteinase inhibitor reduces the performance of two aphid species in artificial diets and transgenic Arabidopsis plants[J].Transgenic Research, 2011, 20（2）：305-319.
[14] 侯汉娜, 欧阳青, 等.抗蚜基因及其转基因植物[J].中国生物工程杂志, 2008, 28（6）：118-124.
[15] DEJM, Lannoo N, Peumans WJ. Plant lectins[J]. Advances in Botanical Research, 2008, 48（3）：107-209.
[16] 喻修道, 唐克轩, 等.用基因枪法获得转异天南星基因aha抗蚜虫小麦[J].作物学报, 2012, 38（8）：1538-1543.
[17] PW, Ellington JJ. Comparison of Capsicum annuum and C. pubescens for antixenosis as a means of Aphid resistance[J]. HortScience, 1996, 31（6）：1017-1018.
[18] VA, Powell KS, Gatehouse AMR, et al. Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphids[J]. Transgenic Research, 1995, 4（1）：18-25.
[19] 田芳, 陈孝, 等. 转基因抗虫小麦中sgna 基因的遗传分析及抗虫性鉴定[J].作物学报, 2004, 30（5）：475-480.
[20] 田芳, 陈孝, 等. 转GNA基因小麦新株系的分子检测和抗蚜虫性鉴定[J].麦类作物学报, 2005, 25（3）：7-10.
[21] 李群, 李冠.雪花莲凝集素基因转化新疆甜瓜抗蚜虫的研究[J].新疆农业科学, 2009, 46（3）：556-560.
[22] 甘敬, 尹伟伦, 等. 国槐转雪花莲凝集素基因及抗蚜性[J].吉林科学, 2010, 46（2）：51-56.
[23] 刘志铭, 刘德璞, 等. CrylA（a）-Pta双价抗虫基因转化粳稻及二化螟抗性评估[J].分子植物育种, 2006, 4（3）：345-350.
[24] 潘映红, 张淑香, 等. 从掌叶半夏和半夏中发现对几种蚜虫有致死活性的蛋白[J].中国农业科学, 1997, 30（2）：94-96.
[25] JH, Zhao XY, Liao ZH, et al. Cloning and molecular characterization of a novel lectin gene from Pinellia ternate[J]. Cell Research, 2003, 13（10）：301-308.
[26] Y, Wei Z. Increased oriental armyworm and aphid resistance in transgenic wheat stably expressing Bacillus thuringiensis（Bt）endotoxin and Pinellia ternate agglutinin（PTA）[J]. Plant Cell Tissue and Organ Culture, 2008, 94（1）：33-44.
[27] 董文琦, 党志红, 等. 半夏凝集素基因克隆及其对桃蚜的抗性研究[J]. 南京农业大学学报, 2010, 33（2）：45-50.
[28] ZM, Yan HB, Pan WL, et al. Transform of an ectopically expressed bulb lectin gene from Pinellia pedatisecta into tobacco plants conferring resistance to aphids[J]. Australian Journal of Crop Science, 2012, 6（5）：904-911.
[29] J, Zhou SG, Liu XJ, et al. cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta[J]. Journal of Biosciences, 2007, 32（2）：241-249.
[30] A, Datta A. Molecular cloning of a gene encoding a seed-specific protein with nutritionally balanced amino acid composition from Amaranthus[J]. Plant Biology, 1992, 89（24）：11774-11778.
[31] HN, Zhou YG, Zhang ZS, et al. Effects of transgenic tobacco plants expressing ACA gene from Amaranthus caudatus on the population development of Myzus persicae[J]. Acta Botanica Sinica, 2004, 46（9）：1100-1105.
[32] J, Luo X, Guo H, et al. Transgenic cotton, expressing Amaranthus caudatus agglutinin, confers enhanced resistance to aphids[J]. Plant Breeding, 2006, 125（4）：390-394.
[33] I, Saha P, Majumder P, et al. The efficacy of a novel insecticidal protein. Allium sativum leaf lectin（ASAL）, against homopteran insects monitored in transgenic tobacco[J]. Plant Biotechnology Journal, 2005, 3（6）：601-611.
[34] A, Broeders S, De GH, et al. Expression of garlic leaf lectin under the control of the phloem-specific promoter Asusl from Arabidopsis thaliana protects tobacco plants against the tobacco aphid（Myzus nicotianae）[J]. Pest Management Science, 2007, 63（12）：1215-1223.
[35] D, Sarkar A, Mondal HA, et al. Tissue specific expression of potent insecticidal, Allium sativum leaf agglutinin（ASAL）in important pulse crop, chickpea（Cicer arietinum L.）to resist the phloem feeding Aphis craccivora[J]. Transgenic Research, 2009, 18（4）：529-544.
[36] N, Nardon C, Febvay G, et al. Binding of the insecticidal lectin Concanavalin A in pea aphid, Acyrthosiphon pisum（Harris）and induced effects on the structure of midgut epithelial cells[J]. Journal of Insect Physiology, 2004, 50（12）：1137-1150.
[37] 转基因抗蚜小麦抗麦长管蚜效果的评价技术研究[D]. 北京：中国农业科学院, 2012.
[38] AMR, Davidson GM, Stewart JN, et al. Concanavalin a inhibits development of tomato moth（Lacanobia oleracea）and peach-potato aphid（Myzus persicae）when expressed in transgenic potato plants[J]. Molecular Breeding, 1999, 5（2）：153-165.
[39] 田颖川, 莽克强, 等. 苋菜凝集素基因的克隆及在转基因烟草中抗蚜性的研究[J].生物工程学报, 2002, 17（3）：34-39.
[40] 魏源文, 吕维莉, 等. MAR序列介导野苋菜凝集素基因在白菜中的表达[J]. 园艺学报, 2007, 34（2）：381-386.
[41] S, Venkateswari J, Kirti PB, et al. Transgenic indian mustard（Brassica juncea）with resistance to the mustard aphid（Lipaphis erysimi Kalt.）[J]. Plant Cell Reports, 2002, 20（10）：976-981.
[42] 吴伯良, 曾仲奎, 等. 海芋凝集素的鉴定及性质研究[J]. 暨南大学学报, 1998, 19（3）：89-93.
[43] YR, Wang J, Huang BQ, et al. Molecular cloning of a lectin cDNA from Alocasia macrorrhiza and prediction of its characteristics[J]. Journal of Plant Physiology and Molecular Biology, 2006, 32（6）：634-642.
[44] 黄炳球, 侯学文.海芋凝集素对豆蚜淀粉酶、蛋白酶活性的影响[J].广东农业科学, 2010, 10（3）：103-105.
[45] M, Coleman AD, Maffei ME, et al. Silencing of aphid genes by dsRNA feeding from plants[J]. PloS One, 2011, 6（10）：117-128.
[46] JJ, Zeng FR. Plant-mediated RNAi of a gap gene-enhanced tobacco tolerance against the Myzus persicae transgenic research[J]. Transgenic Research, 2014, 23（1）145-152.
[47] GY, Sun XF, Zhang YL, et al. Molecular cloning and characterizationof a prenyltransferase from the cotton aphid, Aphis gossypii[J]. Insect Biochem Mol Biol, 2010, 40（7）：552-561.
[48] 方丽平, 张亚楠, 等. 棉花抗蚜性与苯丙氨酸解氨酶活性的研究[J]. 昆虫知识, 2008, 45（3）：422-425.
[49] Y, Wang Y, Bi JL, et al. Constitutive and induced activities of defense-related enzymes in aphid-resistant and aphid-susceptible cultivars of wheat[J]. J Chem Ecol, 2009, 35（2）：176-182.
[50] XD, Jones HD, Ma YZ, et al.（E）-β-farnesene synthase genes affect aphid（Myzus persicae）infestation in tobacco（Nicotiana tabacum）[J]. Funct Integr Genomics, 2012, 12（1）：207-213.
[51] MH, Birkett MA, Bruce TJA, et al. Aphid alarm pheromone produced by transgenic plants affects aphid and parasitoid behavior[J].Proceedings of the National Academy of Sciences of the USA, 2006, 103（27）：10509-10513.
[52] PF, Lagrimini LM, Carozzi N, et al. Advances in insect control：the role of transgenic plants[C]. Taylor & Francis, 1997：1195-2231.
[53] L, Martinez M, Alfageme F, et al. A barley cysteine-proteinase inhibitor reduces the performance of two aphid species in artificial diets and transgenic Arabidopsis plants[J]. Transgenic Research, 2011, 20（2）：305-319.
[54] J, Wu YQ, Xu WG, et al. The impact of transgenic wheat expressing GNA（snowdrop lectin）on the aphids sitobion avenae, schizaphis graminum, and Rhopalosiphum padi[J]. Entomological Society of America, 2011, 40（3）：743-748.
[55] J, Babendreier D, Wackers FL. Consumption of snowdrop lectin（Galanthus nivalis agglutinin）causes direct effects on adult parasitic wasps[J]. Oecologia, 2003, 134（4）：528-536.
[56] PAM, Wackers FL, Woodring JR, et al. Snowdrop lectin（Galanthus nivalis agglutinin）in aphid honeydew negatively affects survival of a honeydew-consuming parasitoid[J]. Agricultural and Forest Entomology, 2009, 11（2）：161-173.
[57] A, Griffiths S, Palacias N, et al. Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV35S promoter and confirms the predominance of microhomology mediat-ed recombination[J]. Plant Journal, 1999, 17（6）：591-601.
[58] N, Broido S, Soreq H, et al. Efficient functioning of plant promoters and poly（A）sites in Xenopus oocytes[J]. Nucleic Acids Research, 1989, 17（19）：7891-7903.
[59] MD, Cheng WY, Summers HE, et al. Alarm pheromone habituation in Myzus persicae has fitness consequences and causes extensive gene expression changes[J]. National Academy of Sciences, 2010, 107（33）：14673-14678.
[60] 赵存友, 周岩, 等. 雪花莲凝集素基因（gna）的改造及其抗蚜性[J]. 植物学报, 2001, 48（6）：592-597.
[61] HM, Guo HN, Jia YT, et al. The effect of TMV-RNA un-translation region on the expression lever of foreign gene in en-tire plant[J]. Chin Sci Bull, 2000, 45（6）：617-622.
[62] 彭爱红, 何永睿, 等. 异源韧皮部特异启动子在转基因枳中的表达[J]. 园艺学报, 2014, 41（1）：1-8.
[63] 秦红敏, 田颖川. 杨树皮储存蛋白基因启动子的克隆和功能研究[J]. 林业科学, 1999, 35（5）：46-50.
[64] E, Audsley N, Gatehouse JA, et al. Fusion proteins containing neuropeptides as novel insect control agents：snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion[J]. Insect Biochemistry and Molecular Biology, 2002, 32（12）：1653-1661.
[65] WJ, Fouquaert E, Jauneau A, et al. The liverwort Marchantia polymorpha expresses orthologs of the fungal Agaricus bisporus agglutinin family[J]. Plant Physiology, 2007, 144（2）：637-647.