Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (10): 111-116.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0491

• Technique • Previous Articles     Next Articles

Strategies for Evaluating the Effects of Transgenic Crops on Soil Microbial Diversity

LIANG Jin-gang, ZHANG Xiu-jie   

  1. Development Center of Science and Technology,Ministry of Agriculture,Beijing 100122
  • Received:2017-06-11 Online:2017-10-29 Published:2017-10-29
  • Contact: 张秀杰,女,副研究员,研究方向:转基因生物安全评价与检测;E-mail:zhxj7410@sina.com

Abstract: Microorganisms are the important components of the soil ecosystem. Soil microbial diversity is crucial to the maintenance of ecosystem functioning as soil microorganisms influence many ecosystem processes and drive biogeochemical cycles. Thus,it becomes a hot topic about the potential risks of transgenic crops to soil microorganisms and their effects on soil ecosystem. With the development of modern biotechnologies,the analysis methods for soil microbial diversity have been developed from the traditional separation culture to study microorganisms in the whole soil microecosystem. However,due to the various characteristics of soil microorganisms(such as majority being non-cultivable,tiny bulk,community quorum sensing,etc.)and the detection limitations of instruments and equipment,there are some disadvantages while only using single research method,and other methods should be combined to study microbial diversity in soil ecosystem. So far,the study on soil microbial diversity mainly includes species diversity,functional diversity,structural diversity and genetic diversity,there are a lot of methods used to study the effects of transgenic crops on the diversity of soil microorganisms. In this review,we summarize these methods,and finally the strategies for the future study are proposed.

Key words: transgenic crops, soil microorganisms, diversity, strategies