Cloning of Chalcone Isomerase Gene from Potato Onion and Plant Expression Vector Construction

Abstract

Abstract: To clone and analyse the function of the potato onion chalcone isomerase gene, we generated CHI over-expression construct and RNAi construct. Full-length CHI gene is 743 bp, and the GenBank accession number is KJ489062. Results showed that, CHI gene open reading frame contain 633 bp, translating into 210 amino acids. Full-length CHI gene and the fragment of CHI gene for silencing were amplified by PCR on complementary DNA（cDNA）, then PCR fragment was transferred to the binary vector under the control of 35S.

Key words: Potato onion, Chalcone isomerase, Gene cloning, Vetor construction

Liu Dan, Wu Fengzhi. Cloning of Chalcone Isomerase Gene from Potato Onion and Plant Expression Vector Construction[J]. Biotechnology Bulletin, 2014, 0(10): 88-93.

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