Study on Antibacterial Effects of Fat-soluble Compositions in Nostoc commune

Abstract

Abstract: The antibacterial effects of fat-soluble compositions from Nostoc commune Vauch. were studied in order to contribute to diverse nutritional and medicinal values of N. commune. Soxhlet extraction method was used to extract crude fats from N. commune, and antimicrobial activities of the liposoluble compounds and fractions isolated by column chromatography were assayed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas stutzeri, Asparagillus niger and Saccharomyces cerevisiae. The results showed fat-soluble compositions were about 2.62% in N. commune. Ethanol elution fractions showed most greatly antimicrobial activities against six bacterial stains while its contents was 32% of crube fats, the benzene fraction with the yeild of 14% in crube fats had worse antimicrobial activities than ethanol elution fractions, and the petroleum ether extract was not inhibitory to six bacterial stains with the yeild of 54% in crube fats. Fat-soluble compositions had most strongly inhibitory effect on E.coli with IC₅₀ 38.75 mg/mL and MIC 64.0 mg/mL and they had least inhibitory effect on Pseudomonas stutzeri with IC₅₀ 89.58 mg/mL and MIC 106.5 mg/mL. Fat-soluble compositions showed antibacterial effects against six bacterial stains：Escherichia coli > Staphylococcus aureus > Bacillus subtilis > Asparagillus niger > Saccharomyces cerevisiae > Pseudomonas stutzeri.

Key words: Nostoc commune, Vauch. Fat-soluble compositions, Antibacterial

DiaoYi, Yang Zujun,. Study on Antibacterial Effects of Fat-soluble Compositions in Nostoc commune[J]. Biotechnology Bulletin, 2014, 0(4): 83-86.

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