Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Wheat Varieties(Lines) in Regional Test of Hebei

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.034

Abstract

Abstract: In order to provide a reference for improving and innovating breeding materials, we used forty two pairs of SSR primers to construct DNA fingerprints and detect genetic diversity among 70 wheat varieties(lines), which participated in regional test of Hebei in 2009-2013. The results showed that 303 allelic were revealed on 42 SSR loci, 7.21 alleles were detected for each SSR locus, and the average PIC(polymorphism information content)value was 0.69. Moreover, the average gene diversity was 0.73, and the genetic similarity varied from 0.05 to 1.00 with the average of 0.28. These results indicated that there is a large genetic difference between the wheat varieties(lines)in regional test of Hebei. The cluster analysis divided the 70 wheat varieties(lines)into four groups and six subgroups by UPGMA method. These results show that the wheat varieties(lines)have similar genetic background in the same breeding institute or region. We should strengthen the introduction and utilization of foreign wheat germplasm resources.

Key words: wheat, SSR markers, DNA fingerprinting, genetic varieties

Li Hongbo, Pang Binshuang, Liu Lihua, Liu Yangna, Zhao Changping, Chen Jingtang. Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Wheat Varieties(Lines) in Regional Test of Hebei[J]. Biotechnology Bulletin, 2015, 31(6): 93-99.

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