Abstract: This work is to characterize cadherin 5（CDH5）gene of Schizothorax prenanti. Bioinformatics tools were used to analyze the gene sequence of CDH5,and real-time PCR to detect the expression variation of CDH5 gene at 0 h,24 h,and 48 h of S. prenanti infected with Aeromomas sobria. The full-length cDNA of CDH5 was 4 825 bp,ORF was 2 313 bp,and encoded 770 amino acids. Its molecular weight was 85.19 kD and pI was 5.01. The CDH5 protein had a signal peptide sequence,and its secondary structure was mainly composed of random coil,extend strand,and α-helix. Multiple alignment analysis revealed that CDH5 shared 74% homology with Danio rerio,low similarity with Homo sapiens（43%）. CDH5 were expressed in all detected tissues after A. sobria infection,the expression of CDH5 in spleen at 24 h was significantly higher than at 0 h and 48 h（P < 0.05）,also the expression of CDH5 in the liver,kidney,and muscle at 48 h higher than at 24 h（P < 0.05）,and significantly higher expression of CDH5 at 48 h in heart and intestines than at 0 h（P < 0.05）. It indicated that CDH5 of S. prenanti might be involved in immune response to A. sobria infection,laying a foundation for the further study on the functions of S. prenanti.

Key words: CDH5, Schizothorax prenanti, tissue expression, Aeromomas sobria