Morphological Identification and Antibacterial Activity on Metabolites of Fungi Isolated from Ascidean

doi:10.13560/j.cnki.biotech.bull.1985.2018-0173

Abstract

Abstract:

To seek new species of marine fungi with high antibacterial activity,ascidean was used as study material,and 14 fungi were isolated by plate spreading method,and their morphological identification and preliminary screening of their antibacterial activity was conducted. The results indicated that 14 fungal strains were isolated from ascidian,and preliminarily identified as Penicillium sp.,Aspergillus sp.,Cladosporium sp.,and Tritirachium sp. based on the morphological observation. Total 11 fungal strains of 14 presented antibacterial activity,and 4 strains of Asc-2-4（Penicillium sp.）,Asc-2-12（Penicillium sp.）,Asc-1-1（Penicillium sp.）,and Asc-2-1（Aspergillus sp.）had broad-spectrum and strong antibacterial activity. The antibacterial activity of Asc-2-12（Penicillium sp.）was the most obvious,with the diameter of maximum inhibition circle reaching 21.91 mm. Symbiotic fungal strains from ascidian are potential antibacterial resources and Asc-2-4,Asc-2-12,Asc-1-1,and Asc-2-1 have potential prospect to be further investigated.

Key words: ascidean, symbiotic fungi, morphological identification, antibacterial activity

LUO Guo-cong, HUANG Yun-yi, CHAI Hui-zi, LEI Xiao-ling, NIE Fang-hong. Morphological Identification and Antibacterial Activity on Metabolites of Fungi Isolated from Ascidean[J]. Biotechnology Bulletin, 2018, 34(9): 244-248.

References

[1] 李立明, 胡志钰, 黄耀坚, 等. 海洋动植物共附生放线菌抗真菌活性菌株的生态分布[A]. 海洋通报, 2002, 3(21):27-31.
[2] Zhao C, Zhu T, Zhu W.New marine natural products of microbial origin from 2010 to 2013[J]. Chinese Journal of Organic Chemistry, 2013, 33(6):1195-1234.
[3] 徐年军, 严小军. 海洋微生物的化学生态学研究进展[J]. 应用生态学报, 2006(12):2436-2440.
[4] 郑成兴. 中国沿海海鞘的物种多样性[J]. 生物多样性, 1995, 3(4):201-205.
[5] 樊成奇, 陆亚男, 缪宇平, 等. 东海水产养殖区七种海鞘优势种相关活性物质研究进展与利用前景[J]. 海洋渔业, 2009, 31(2):207-214.
[6] 缪承杜, 洪葵. 真菌分类技术的研究进展[J]. 安徽农业科学, 2007, 35(22):6695-6697.
[7] 柴慧子, 黄蕴怡, 等. 海鞘中产油脂共附生真菌的筛选及脂肪酸组成成分分析[J]. 现代食品科技, 2016(12):287-291.
[8] 钱存柔,黄仪秀, 等. 微生物学实验[M]. 北京:北京大学出版社, 1985.
[9] 杨宇容, 徐丽华, 李启任, 等. 放线菌分离方法的研究I. 抑制剂的选择[J], 微生物学通报, 1985, 22(2):88-91.
[10] 魏景超. 真菌鉴定手册[M]. 上海:上海科学技术出版社, 1979.
[11] 齐祖同, 孙华忠. 中国真菌志. 曲霉属及其相关有性型[M]. 第5卷, 北京:科学出版社, 1997.
[12] 孔华忠. 中国真菌志. 青霉属及其相关有性形属[M]第35卷 . 北京:科学出版社, 2007.
[13] 张中义. 中国真菌志. 枝孢属黑星孢属梨孢属[M]. 第14卷. 北京:科学出版社, 2003.
[14] 中国科学院微生物研究所《常见与常用真菌》编写组. 常见与常用真菌[M]. 北京:科学出版社, 1973.
[15] 崔迪. 威海海域海鞘来源可培养细菌多样性和活性菌株筛选[D]. 哈尔滨:哈尔滨工业大学, 2014.
[16] Abedi E, Sahari MA.Long-chain polyunsaturated fatty acid sources and evaluation of their nutritional and functional properties[J]. Food Science & Nutrition, 2014, 2(5):443-463.
[17] Papanikolaou S, Beopoulos A, Koletti A, et al.Importance of the methyl-citrate cycle on glycerol metabolism in the yeast Yarrowia lipolytica[J]. J Biotechnol, 2013, 168(4):303-314.
[18] 苗婷婷, 邢翔, 等. 柄海鞘共附生细菌的分离培养与系统发育多样性研究[J]. 海洋科学进展, 2012, 30(1):111-118.
[19] 裴召苓. 海鞘来源三株微生物的次级代谢产物及其生物活性研究[D]. 哈尔滨:哈尔滨工业大学, 2016.
[20] 李贺, 林学政, 何培青, 等. 南极抗细菌活性菌株的筛选及系统发育分析[J]. 微生物学通报, 2011(2):264-269.
[21] 韩妍妍, 张亚娟, 王维娜, 等. 海洋微生物是开发海洋药物的重要资源[J]. 海洋科学, 2002, 26(9):7-12.