Preparation of Cross-Linked Enzyme Aggregates and Its Application in Laccase Immobilization

doi:10.13560/j.cnki.biotech.bull.1985.2018-0935

Abstract

Abstract: Laccase is a copper-containing polyphenol oxidase with high catalytic efficiency. Due to its broad substrate range,laccase has been widely used in many industrial fields. Cross-linked enzyme aggregates（CLEAs）technology is a novel carrier-free enzyme immobilization approach that could be simply performed using crude enzyme at low cost. The CLEAs usually have high stability and can be reused in multiple cycles. In this paper,we first analyzed the factors affecting enzyme activity during the CLEAs preparation and the related improving strategies,and then summarized the application of cross-linked laccase aggregates in pollutant degradation,with the aim of laying a foundation for further research and application of immobilized laccase.

Key words: immobilized enzyme, laccase, cross-linked enzyme aggregates, pollutant degradation

HAN Shu-ran, LU Lei. Preparation of Cross-Linked Enzyme Aggregates and Its Application in Laccase Immobilization[J]. Biotechnology Bulletin, 2019, 35(3): 164-170.

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