Advances and Prospects of Synthetic Biology in Lactic Acid Bacteria

doi:10.13560/j.cnki.biotech.bull.1985.2019-0086

Abstract

Abstract: As the traditional food-grade microorganism,lactic acid bacteria have been applied in food industry,health care,and clinical medicine for a long time. With the need for special functions of lactic acid bacteria increasing,the traditional screening strain method gradually becomes the bottleneck of hindering the development of lactic acid industry due to its complex technology,long cycle,and low success rate. Fortunately,synthetic biology provides new opportunities for developing novel functional lactic acid bacteria strains by modifying cells to meet the designer’s demands with the method of introducing a gene network of specific functions into the genome. Firstly,this paper summarizes the characteristics of lactic acid bacteria and explores the advantages of lactic acid bacteria as a synthetic biological chassis. Then,this paper reviews the current situation of lactic acid bacteria synthesis biology in component design,vector selection,transformation methods and gene editing techniques. Finally,this paper summarizes the application of engineered lactic acid bacteria in disease diagnosis and treatment,food quality improvement and bioenergy,and discusses the technological breakthroughs promoting the further applications,which would be guidance for the development of synthetic biology in lactic acid bacteria.

Key words: lactic acid bacteria, synthetic biology, intestinal regulation, chassis

LIU Yang-er, GUO Ming-zhang, DU Ruo-xi, HE Xiao-yun, HUANG Kun-lun, XU Wen-tao. Advances and Prospects of Synthetic Biology in Lactic Acid Bacteria[J]. Biotechnology Bulletin, 2019, 35(8): 193-204.

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