Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 88-95.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0749
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ZHA Xing-qin1(), YANG Ming-hua2, LI Yong-neng3, ZHAO Su-mei2(), HUANG Ying2()
Received:
2020-06-18
Online:
2021-02-26
Published:
2021-02-26
Contact:
ZHAO Su-mei,HUANG Ying
E-mail:chaxingqin-jk@163.com;zhaosm2009@126.com;410457491@qq.com
ZHA Xing-qin, YANG Ming-hua, LI Yong-neng, ZHAO Su-mei, HUANG Ying. Study on the Regulation of Leptin-mediated JAK/STAT Signal Pathway on Lipid Metabolism in Porcine Subcutaneous Preadipocyte[J]. Biotechnology Bulletin, 2021, 37(2): 88-95.
目的基因 | 引物序列(5'-3') | 退火温度/℃ | 产物大小/bp | GenBank登录号 |
---|---|---|---|---|
18S rRNA | F:TAGAGTCACCAAAGCCGC | 55 | 194 | NR_002170.3 |
R:TCAGTTATGGTTCCTTTG | ||||
LepR | F:GTGATAACTGCATTTGACTTGGC | 62 | 271 | NM001024587 |
R:CTGCAATGTTGTCTGCATGTACAG | ||||
JAK2 | F:ATTTGAAGACCGAGACCC | 60 | 272 | NM_214113.1 |
R:TTAGATTACGCCGACCAG | ||||
STAT3 | F:CAGTAAGGAGAGGGAGCG | 56 | 254 | NM_001044580 |
R:AGAGGTAGACCAGCGGAG | ||||
CPT-1 | F:ATGGTGGGCGACTAACT | 60 | 321 | AY181062 |
R:TGCCTGCTGTCTGTGAG | ||||
ACOX1 | F:CGGGCACGGCTATTCTCAC | 56 | 193 | NM_001101028 |
R:CTGGCTGGGCAGGTCATTC | ||||
PGC-1α | F:CCCAGTCTGCGGCTATTTG | 56 | 282 | AB106108.1 |
R:GGGATGACCGAAGTGCTTG |
目的基因 | 引物序列(5'-3') | 退火温度/℃ | 产物大小/bp | GenBank登录号 |
---|---|---|---|---|
18S rRNA | F:TAGAGTCACCAAAGCCGC | 55 | 194 | NR_002170.3 |
R:TCAGTTATGGTTCCTTTG | ||||
LepR | F:GTGATAACTGCATTTGACTTGGC | 62 | 271 | NM001024587 |
R:CTGCAATGTTGTCTGCATGTACAG | ||||
JAK2 | F:ATTTGAAGACCGAGACCC | 60 | 272 | NM_214113.1 |
R:TTAGATTACGCCGACCAG | ||||
STAT3 | F:CAGTAAGGAGAGGGAGCG | 56 | 254 | NM_001044580 |
R:AGAGGTAGACCAGCGGAG | ||||
CPT-1 | F:ATGGTGGGCGACTAACT | 60 | 321 | AY181062 |
R:TGCCTGCTGTCTGTGAG | ||||
ACOX1 | F:CGGGCACGGCTATTCTCAC | 56 | 193 | NM_001101028 |
R:CTGGCTGGGCAGGTCATTC | ||||
PGC-1α | F:CCCAGTCTGCGGCTATTTG | 56 | 282 | AB106108.1 |
R:GGGATGACCGAAGTGCTTG |
指标 | 对照组 | Leptin组 |
---|---|---|
甘油三酯(mmol/L) | 0.15±0.02a | 0.07±0.01b |
游离脂肪酸(μmol/gprot) | 1.16±0.13a | 0.94±0.09a |
指标 | 对照组 | Leptin组 |
---|---|---|
甘油三酯(mmol/L) | 0.15±0.02a | 0.07±0.01b |
游离脂肪酸(μmol/gprot) | 1.16±0.13a | 0.94±0.09a |
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