Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (9): 68-76.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1104
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JIANG Fu-gui(), CHENG Hai-jian, WEI Chen, ZHANG Zhao-kun, SU Wen-zheng, SHI Guang, SONG En-liang()
Received:
2021-08-26
Online:
2021-09-26
Published:
2021-10-25
Contact:
SONG En-liang
E-mail:fgjiang2017@163.com;enliangs@126.com
JIANG Fu-gui, CHENG Hai-jian, WEI Chen, ZHANG Zhao-kun, SU Wen-zheng, SHI Guang, SONG En-liang. Effects of Addition Amount of Molasses on the Fermentation Quality and Microbial Diversity of Hybrid Broussonetia papyrifera L. Vent Silage[J]. Biotechnology Bulletin, 2021, 37(9): 68-76.
项目Item | 含量Content/% |
---|---|
干物质DM | 28.73 |
粗蛋白CP | 17.69 |
中性洗涤纤维NDF | 44.80 |
酸性洗涤纤维ADF | 23.07 |
酸性洗涤木质素ADL | 8.70 |
粗灰分Ash | 11.70 |
粗脂肪EE | 6.49 |
水溶性碳水化合物WSC | 1.27 |
Table 1 Nutritional composition of hybrid B. papyrifera L. Vent(dry matter basis)
项目Item | 含量Content/% |
---|---|
干物质DM | 28.73 |
粗蛋白CP | 17.69 |
中性洗涤纤维NDF | 44.80 |
酸性洗涤纤维ADF | 23.07 |
酸性洗涤木质素ADL | 8.70 |
粗灰分Ash | 11.70 |
粗脂肪EE | 6.49 |
水溶性碳水化合物WSC | 1.27 |
项目Item | 不同处理组Treatment | 标准误SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
干物质DM/% | 24.41b | 26.83a | 28.02a | 28.32a | 0.466 | 0.001 | <0.001 | 0.027 | 0.898 |
粗蛋白质CP/% | 15.18b | 16.38ab | 16.98ab | 17.64a | 0.360 | 0.047 | 0.020 | 0.889 | 0.282 |
中性洗涤纤维NDF/% | 37.56a | 35.42a | 32.13b | 31.39b | 0.718 | <0.001 | <0.001 | 0.034 | 0.237 |
酸性洗涤纤维ADF/% | 20.88a | 20.48a | 19.41a | 17.41b | 0.403 | <0.001 | <0.001 | 0.490 | 0.658 |
酸性洗涤木质素ADL/% | 5.97 | 6.40 | 5.35 | 4.64 | 0.294 | 0.161 | 0.149 | 0.612 | 0.321 |
粗灰分Ash/% | 12.63a | 11.80b | 11.78b | 11.53b | 0.139 | 0.009 | 0.004 | 0.078 | 0.217 |
粗脂肪EE/% | 6.96 | 7.98 | 7.67 | 7.58 | 0.367 | 0.828 | 0.733 | 0.510 | 0.592 |
水溶性碳水化合物WSC/% | 0.54c | 0.53c | 0.79b | 1.08a | 0.064 | <0.001 | <0.001 | 0.303 | 0.123 |
Table 2 Effects of addition amount of molasses on nutrient contents of hybrid B. papyrifera L. Vent silage(dry matter basis)
项目Item | 不同处理组Treatment | 标准误SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
干物质DM/% | 24.41b | 26.83a | 28.02a | 28.32a | 0.466 | 0.001 | <0.001 | 0.027 | 0.898 |
粗蛋白质CP/% | 15.18b | 16.38ab | 16.98ab | 17.64a | 0.360 | 0.047 | 0.020 | 0.889 | 0.282 |
中性洗涤纤维NDF/% | 37.56a | 35.42a | 32.13b | 31.39b | 0.718 | <0.001 | <0.001 | 0.034 | 0.237 |
酸性洗涤纤维ADF/% | 20.88a | 20.48a | 19.41a | 17.41b | 0.403 | <0.001 | <0.001 | 0.490 | 0.658 |
酸性洗涤木质素ADL/% | 5.97 | 6.40 | 5.35 | 4.64 | 0.294 | 0.161 | 0.149 | 0.612 | 0.321 |
粗灰分Ash/% | 12.63a | 11.80b | 11.78b | 11.53b | 0.139 | 0.009 | 0.004 | 0.078 | 0.217 |
粗脂肪EE/% | 6.96 | 7.98 | 7.67 | 7.58 | 0.367 | 0.828 | 0.733 | 0.510 | 0.592 |
水溶性碳水化合物WSC/% | 0.54c | 0.53c | 0.79b | 1.08a | 0.064 | <0.001 | <0.001 | 0.303 | 0.123 |
项目Item | 不同处理组Treatments | 标准差 SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
pH | 5.25a | 4.66b | 4.24c | 4.08d | 0.118 | <0.001 | <0.001 | <0.001 | 0.776 |
乳酸Lactic acid /(g·kg-1) | 21.64b | 46.98ab | 59.64a | 73.67a | 6.774 | 0.024 | 0.002 | 0.120 | 0.611 |
乙酸Acetic acid /(g·kg-1) | 24.96 | 26.04 | 24.09 | 22.72 | 0.649 | 0.342 | 0.131 | 0.595 | 0.401 |
丙酸Propionic acid /(g·kg-1) | 1.10a | 0.96ab | 0.52b | 0.44b | 0.104 | 0.043 | 0.010 | 0.380 | 0.358 |
丁酸Butyric acid /(g·kg-1) | 11.01a | 0.62b | 0.45b | 0.43b | 1.442 | 0.004 | 0.005 | 0.008 | 0.147 |
氨态氮/总氮NH3-N/TN /% | 13.40a | 7.02b | 5.18b | 2.53c | 1.092 | <0.001 | <0.001 | 0.003 | 0.150 |
Table 3 Effects of addition amount of molasses on the fermentation quality of hybrid B. papyrifera L. Vent silage(dry matter basis)
项目Item | 不同处理组Treatments | 标准差 SEM | P值P value | ||||||
---|---|---|---|---|---|---|---|---|---|
CK | M5 | M10 | M20 | T | L | Q | C | ||
pH | 5.25a | 4.66b | 4.24c | 4.08d | 0.118 | <0.001 | <0.001 | <0.001 | 0.776 |
乳酸Lactic acid /(g·kg-1) | 21.64b | 46.98ab | 59.64a | 73.67a | 6.774 | 0.024 | 0.002 | 0.120 | 0.611 |
乙酸Acetic acid /(g·kg-1) | 24.96 | 26.04 | 24.09 | 22.72 | 0.649 | 0.342 | 0.131 | 0.595 | 0.401 |
丙酸Propionic acid /(g·kg-1) | 1.10a | 0.96ab | 0.52b | 0.44b | 0.104 | 0.043 | 0.010 | 0.380 | 0.358 |
丁酸Butyric acid /(g·kg-1) | 11.01a | 0.62b | 0.45b | 0.43b | 1.442 | 0.004 | 0.005 | 0.008 | 0.147 |
氨态氮/总氮NH3-N/TN /% | 13.40a | 7.02b | 5.18b | 2.53c | 1.092 | <0.001 | <0.001 | 0.003 | 0.150 |
Fig. 1 Alpha diversity analysis of hybrid B. papyrifera L. Vent fresh material and silage The histogram with different superscripts letters among treatments refer to significantly different(P < 0.05). CK:control group.M5:5 g/kg molasses group. M10:10 g/kg molasses group. M20:20 g/kg molasses group. The same below
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