Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (10): 216-225.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0099

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Whole Genome Sequencing and Comparative Genomic Analysis of a High-yield Lipase-producing Strain WCO-9

ZHANG Ze-ying1,3(), FAN Qing-feng1,3, DENG Yun-feng1,3, WEI Ting-zhou1,3, ZHOU Zheng-fu2, ZHOU Jian1,3, WANG Jin2, JIANG Shi-jie1,3()   

  1. 1. College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010
    2. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081
    3. Engineering Research Center of Biomass Materials,Ministry of Education,Southwest University of Science and Technology,Mianyang 621010
  • Received:2021-01-21 Online:2022-10-26 Published:2022-11-11
  • Contact: JIANG Shi-jie E-mail:1148315372@qq.com;sjjiang0406@swust.edu.cn

Abstract:

Acinetobacter junii WCO-9 is a high-yield lipase-producing strain isolated from oil-contaminated soil. The lipase produced by this strain has significantly better hydrolysis activity than that of the control strain ATCC 17908. In order to explore the efficient enzyme production mechanism of WCO-9 strain and to discover specific lipase functional genes,ONT(Oxford Nanopore Technologies)was used to complete the whole genome sequencing of WCO-9 strain,and the comparative genomic analysis of the same strain was carried out. The results showed that the genome size of lipase-producing WCO-9 strain was 3.19 Mb,GC content was 38.62%,and there were 2 929 coding genes,including 11 lipase-coding genes(1 specific gene)and 3 lipase molecular chaperones. The collinearity analysis also revealed that the WCO-9 strain was significantly different from the control strain ATCC 17908. The whole genome sequence analysis of WCO-9 strain will be of great significance for the study of high-efficiency enzyme production mechanism and the discovery of new lipase-specific genes,which provides a theoretical basis for the creation and industrial application of high-yield lipase engineering bacteria in the future.

Key words: lipase, Acinetobacter junii, whole genome sequencing, gene function annotation, comparative genome analysis