Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (2): 10-20.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0542

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Proteomic Analysis of RPP1A Involved in the Seedling Growth of Arabidopsis thaliana

LI Bing-juan1,2(), ZHENG Lu1, SHEN Ren-fang1,2, LAN Ping1,2()   

  1. 1. State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008
    2. University of Chinese Academy of Sciences,Beijing 100049
  • Received:2021-04-23 Online:2022-02-26 Published:2022-03-09
  • Contact: LAN Ping E-mail:libingjuan313@163.com;plan@issas.ac.cn

Abstract:

Ribosomal proteins are not only involved in protein synthesis but also regulate plant growth and development. Arabidopsis thaliana ribosomal phosphoprotein P1(RPP1)family RPP1A deletion mutant rpp1a was used to investigate the effect of RPP1A deletion on the protein profile of seedlings and to reveal the mechanism of its involvement in regulation of seedling growth. Phenotypic analysis revealed that the RPP1A deletion resulted in significant increases in biomass,total leaf surface area,and petiole length of the seedlings compared with wild-type(WT). The label-free proteomic analysis revealed that a total of 280 differentially expressed proteins were identified in the mutant rpp1a-2 compared with the WT,with 98 proteins significantly up-regulated and 182 proteins significantly down-regulated. Differentially expressed proteins were mainly involved in cellular process,metabolic process,response to stimulus,cellular component organization or biogenesis,and oxidation-reduction process,and were significantly enriched in the metabolic pathways of ribosome,photosynthesis,mRNA surveillance pathway,RNA degradation and phenylpropanoid biosynthesis. The A. thaliana rpp1a-2 mutant promotes plant growth by inhibiting the stimulus-response and enhancing photosynthesis.

Key words: Arabidopsis thaliana, ribosomal proteins, seedling growth, proteomics, stimulus response, photosynthesis