Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (5): 100-111.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1557

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Screening,Identification and Broad-spectrum Application of Efficient IAA-producing Bacteria Dissolving Phosphorus and Potassium

ZHANG Hao-xin1(), WANG Zhong-hua1, NIU bing1, GUO Kang1, LIU Lu1, JIANG Ying1(), ZHANG Shi-xiang2()   

  1. 1. College of Resources and Environmental Science,Henan Agricultural University,Zhengzhou 450002
    2. Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001
  • Received:2021-12-16 Online:2022-05-26 Published:2022-06-10
  • Contact: JIANG Ying,ZHANG Shi-xiang E-mail:zhx13243155075@163.com;JY27486@163.com;xuyizhangshix@163.com

Abstract:

We screened the growth-promoting bacteria species from plant rhizosphere and found out their growth-promoting ability under laboratory and field conditions,which may provide a potential bacterial fertilizer for promoting the production and efficiency in agriculture generally. The strains were isolated from rhizosphere soil,the IAA production of these strains was determined by Salkowski colorimetry,and the phosphorus- and potassium-dissolving abilities were determined by molybdenum antimony resistance colorimetry and flame photometry. The high-efficiency growth-promoting strains were identified in this study based on the morphology,physiology and biochemistry and 16S rDNA. The optimal conditions of these strains to produce IAA were explored and further applied to the pot and field experiments for studying its actual application effects. A strain of Bacillus wiedmannii YC9 was selected,it produced 61.71 mg/L of IAA,dissolved 17.57 mg/L of potassium and 98.25 mg/L and 0.64 mg/L of inorganic phosphorus and organic phosphorus. The IAA production of YC9 reached the peak under the conditions that the liquid volume was 150/250 mL,the pH was 7-9,the carbon source was glucose,and the nitrogen source was peptone. In the pot experiment,principal component analysis(PCA)combined with correlation analysis indicated that YC9 promoted the growth of tobacco roots and shoots by increasing IAA and available phosphorus and potassium in the soil. In the field trials of tobacco and wheat,the yield of flue-cured tobacco and the proportion of high-quality tobacco increased by 14.3% and 9.6%,respectively,and the yield of wheat increased by 43.8%,indicating that YC9 had a good application effect in both tobacco and wheat cultivation. YC9 has been identified as a multifunctional growth-promoting bacterium that can produce IAA,hydrolyze phosphorus and potassium. It improves the yield and quality of crops efficiently in the fluvo aquic soil. Our study shows it an outstanding microbial resource of growth-promoting bacterial fertilizer with a broad-spectrum application prospect.

Key words: fluvo aquic soil, rhizosphere growth-promoting bacteria, tobacco, wheat, Bacillus wiedmannii