Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (5): 100-111.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1557
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ZHANG Hao-xin1(), WANG Zhong-hua1, NIU bing1, GUO Kang1, LIU Lu1, JIANG Ying1(), ZHANG Shi-xiang2()
Received:
2021-12-16
Online:
2022-05-26
Published:
2022-06-10
Contact:
JIANG Ying,ZHANG Shi-xiang
E-mail:zhx13243155075@163.com;JY27486@163.com;xuyizhangshix@163.com
ZHANG Hao-xin, WANG Zhong-hua, NIU bing, GUO Kang, LIU Lu, JIANG Ying, ZHANG Shi-xiang. Screening,Identification and Broad-spectrum Application of Efficient IAA-producing Bacteria Dissolving Phosphorus and Potassium[J]. Biotechnology Bulletin, 2022, 38(5): 100-111.
有机质Organic matter/% | 全磷Total P/(g·kg-1) | 有效磷Available P/(mg·kg-1) | 全钾Total K/(g·kg-1) | 有效钾Available K/(mg·kg-1) | pH |
---|---|---|---|---|---|
1.11 | 0.80 | 15.93 | 18.56 | 125.33 | 7.8 |
Table 1 Basic physical and chemical properties of tested soil
有机质Organic matter/% | 全磷Total P/(g·kg-1) | 有效磷Available P/(mg·kg-1) | 全钾Total K/(g·kg-1) | 有效钾Available K/(mg·kg-1) | pH |
---|---|---|---|---|---|
1.11 | 0.80 | 15.93 | 18.56 | 125.33 | 7.8 |
不同条件Different condition | 装液量 Liquid loading/(mL·250 mL-1) | pH | 氮源Nitrogen source | 碳源Carbon source |
---|---|---|---|---|
1 | 25 | 4 | 硝酸钾 | 葡萄糖 |
2 | 50 | 5 | 硫酸铵 | 木糖 |
3 | 75 | 6 | 硝酸铵 | 蔗糖 |
4 | 100 | 7 | 酵母粉 | 果糖 |
5 | 150 | 8 | 谷氨酸 | 甘露醇 |
6 | 9 | 尿素 | 乳糖 | |
7 | 10 | 蛋白胨 | 麦芽糖 |
Table 2 Optimization of conditions for strain growth and IAA production capacity
不同条件Different condition | 装液量 Liquid loading/(mL·250 mL-1) | pH | 氮源Nitrogen source | 碳源Carbon source |
---|---|---|---|---|
1 | 25 | 4 | 硝酸钾 | 葡萄糖 |
2 | 50 | 5 | 硫酸铵 | 木糖 |
3 | 75 | 6 | 硝酸铵 | 蔗糖 |
4 | 100 | 7 | 酵母粉 | 果糖 |
5 | 150 | 8 | 谷氨酸 | 甘露醇 |
6 | 9 | 尿素 | 乳糖 | |
7 | 10 | 蛋白胨 | 麦芽糖 |
有机质 Organic matter/% | 全磷 Total P/(g·kg-1) | 有效磷 Available P/(mg·kg-1) | 全钾 Total K/(g·kg-1) | 有效钾 Available K/(mg·kg-1) | 全氮 Total N/(g·kg-1) |
---|---|---|---|---|---|
1.91 | 0.29 | 2.44 | 19.56 | 30.42 | 0.46 |
Table 3 Basic properties of test soil in wheat field
有机质 Organic matter/% | 全磷 Total P/(g·kg-1) | 有效磷 Available P/(mg·kg-1) | 全钾 Total K/(g·kg-1) | 有效钾 Available K/(mg·kg-1) | 全氮 Total N/(g·kg-1) |
---|---|---|---|---|---|
1.91 | 0.29 | 2.44 | 19.56 | 30.42 | 0.46 |
菌株 Strain | 产IAA能力 IAA content/(mg·L-1) | 解钾能力 Available K content/(mg·L-1) | 解无机磷能力 Available P content/(mg·L-1) | 解有机磷能力 Available P content/(mg·L-1) |
---|---|---|---|---|
YC9 | 61.71 | 17.57 | 98.25 | 0.64 |
Table 4 Various growth promoting abilities of YC9
菌株 Strain | 产IAA能力 IAA content/(mg·L-1) | 解钾能力 Available K content/(mg·L-1) | 解无机磷能力 Available P content/(mg·L-1) | 解有机磷能力 Available P content/(mg·L-1) |
---|---|---|---|---|
YC9 | 61.71 | 17.57 | 98.25 | 0.64 |
项目 Item | 结果 Result | 参考菌株 Reference strain | 项目 Item | 结果 Result | 参考菌株 Reference strain | |
---|---|---|---|---|---|---|
革兰氏染色 Gram stain | + | + | 淀粉水解 Amylohydrolysis test | + | + | |
好氧性试验 Aerobic test | 兼性厌氧 | 兼性厌氧 | 明胶液化 Gelatin liquefaction test | + | + | |
接触酶试验 Catalase | + | + | 硝酸盐还原 Nitrate reduction test | + | + | |
甲基红(M.R)反应 Methyl red test | - | - | 柠檬酸盐利用 Citrate utilization | - | +(55) | |
V-P试验 Voges-Proskauer test | + | + |
Table 5 Physiological and biochemical characteristics of YC9 strain
项目 Item | 结果 Result | 参考菌株 Reference strain | 项目 Item | 结果 Result | 参考菌株 Reference strain | |
---|---|---|---|---|---|---|
革兰氏染色 Gram stain | + | + | 淀粉水解 Amylohydrolysis test | + | + | |
好氧性试验 Aerobic test | 兼性厌氧 | 兼性厌氧 | 明胶液化 Gelatin liquefaction test | + | + | |
接触酶试验 Catalase | + | + | 硝酸盐还原 Nitrate reduction test | + | + | |
甲基红(M.R)反应 Methyl red test | - | - | 柠檬酸盐利用 Citrate utilization | - | +(55) | |
V-P试验 Voges-Proskauer test | + | + |
Fig. 3 Effects of different culture conditions on the IAA production capacity and growth status(OD600)of YC9 strain Different letters indicate the effects of different conditions on the IAA production capacity and growth status of YC9
处理Treatment | IAA/(mg·kg-1) | 速效钾Available K/(mg·kg-1) | 速效磷Available P/(mg·kg-1) |
---|---|---|---|
CK | 0.26±0.04 | 135.00±7.35 | 5.04±0.08 |
YC9 | 0.94±0.05** | 149.00±1.73* | 6.11±0.32* |
Table 6 Effects of inoculating strain YC9 on the soil IAA and available phosphorus and potassium contents after 30 d
处理Treatment | IAA/(mg·kg-1) | 速效钾Available K/(mg·kg-1) | 速效磷Available P/(mg·kg-1) |
---|---|---|---|
CK | 0.26±0.04 | 135.00±7.35 | 5.04±0.08 |
YC9 | 0.94±0.05** | 149.00±1.73* | 6.11±0.32* |
标标Index | 根总长 Root length/cm | 根表面积 Root surface area/cm2 | 根体积 Root volume/cm3 | 根尖数 Number of root tips | 根平均直径 Average root diameter/mm | 根分枝数 Number of root branches | 植株鲜重 Plant fresh weight/g | 株高 Plant height/cm | SPAD | 植株全氮 Plant total N/(g·kg-1) | 植株全磷 Plant total P/(g·kg-1) | 植株全钾 Plant total K/(g·kg-1) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
土壤速效磷 Soil P/(mg·kg-1) | 0.855* | 0.802 | 0.880* | 0.717 | 0.479 | 0.613 | 0.816* | 0.793 | 0.824* | 0.755 | 0.783 | 0.771 |
土壤速效钾 Soil K/(mg·kg-1) | 0.903* | 0.867* | 0.881* | 0.768 | 0.638 | 0.719 | 0.994** | 0.988** | 0.934** | 0.538 | 0.959** | 0.877* |
土壤IAA/(mg·kg-1) | 0.855 | 0.802 | 0.880 | 0.717 | 0.479 | 0.613 | 0.816 | 0.793 | 0.824 | 0.755 | 0.783 | 0.771 |
Table 7 Correlation analysis of changes of tobacco pot soil and tobacco indexes under YC9 treatment
标标Index | 根总长 Root length/cm | 根表面积 Root surface area/cm2 | 根体积 Root volume/cm3 | 根尖数 Number of root tips | 根平均直径 Average root diameter/mm | 根分枝数 Number of root branches | 植株鲜重 Plant fresh weight/g | 株高 Plant height/cm | SPAD | 植株全氮 Plant total N/(g·kg-1) | 植株全磷 Plant total P/(g·kg-1) | 植株全钾 Plant total K/(g·kg-1) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
土壤速效磷 Soil P/(mg·kg-1) | 0.855* | 0.802 | 0.880* | 0.717 | 0.479 | 0.613 | 0.816* | 0.793 | 0.824* | 0.755 | 0.783 | 0.771 |
土壤速效钾 Soil K/(mg·kg-1) | 0.903* | 0.867* | 0.881* | 0.768 | 0.638 | 0.719 | 0.994** | 0.988** | 0.934** | 0.538 | 0.959** | 0.877* |
土壤IAA/(mg·kg-1) | 0.855 | 0.802 | 0.880 | 0.717 | 0.479 | 0.613 | 0.816 | 0.793 | 0.824 | 0.755 | 0.783 | 0.771 |
处理 Treatments | 单叶重Single leaf weight/g | 产量 Yield/g | 上等烟比例 Proportion of superior tabacco/% | ||
---|---|---|---|---|---|
下桔二 (X2F) | 中桔三 (C3F) | 上桔二 (B2F) | |||
CK | 7.2 | 10.7 | 12.5 | 2015.0 | 58.2 |
YC9 | 7.5 | 11.9 | 13.4 | 2302.4 | 63.8 |
Table 8 Effects of YC9 treatment on the yield characters of flue-cured tobacco
处理 Treatments | 单叶重Single leaf weight/g | 产量 Yield/g | 上等烟比例 Proportion of superior tabacco/% | ||
---|---|---|---|---|---|
下桔二 (X2F) | 中桔三 (C3F) | 上桔二 (B2F) | |||
CK | 7.2 | 10.7 | 12.5 | 2015.0 | 58.2 |
YC9 | 7.5 | 11.9 | 13.4 | 2302.4 | 63.8 |
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